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Product Name
Anti-IKK alpha Rabbit antibody
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Description
IKK alpha Rabbit polyclonal antibody
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Tested applications
WB, IHC-P, ICC/IF, IP, FC
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Species reactivity
Human, Mouse, Rat
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthesized peptide derived from human IKK alpha
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Clonality
Polyclonal
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Formulation
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/5000-1/20000
IHC: 1/50-1/200
ICC/IF: 1/50-1/200
IP: 1/50
FC: 1/50
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Validations
Immunohistochemical analysis of paraffin-embedded mouse kidney, using IKK alpha Antibody.
Western blot analysis of extracts from Jurkat and K562 cells using IKKα Rabbit pAb at 1:1000 dilution. Predicted band size: 85kDa. Observed band size: 85kDa.
Western blot analysis of IKK alpha expression in Daudi cell lysate.
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Background
Swiss-Prot Acc.O15111.Plays an essential role in the NF-kappa-B signaling pathway which is activated by multiple stimuli such as inflammatory cytokines, bacterial or viral products, DNA damages or other cellular stresses. Activation of IKK depends upon phosphorylation at Ser177 and Ser181 in the activation loop of IKKβ (Ser176 and Ser180 in IKKα), which causes conformational changes, resulting in kinase activation.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"