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Product Name
Anti-IGFBP-2 / IGFBP2 antibody
- Documents
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Description
Rabbit polyclonal to IGFBP-2 / IGFBP2
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Tested applications
ELISA, WB, IHC-P, IP
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Species reactivity
Cynomolgus IGFBP-2 / IGFBP2
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Alternative names
AI255832 antibody; IBP2 antibody; IBP-2 antibody; IBP-2 antibody; IGF-binding protein 2 antibody; IGF-binding protein 2 antibody; Igfbp-2 antibody; IGFBP-2 antibody; IGF-BP53 antibody; insulin-like growth factor-binding protein 2 antibody; insulin-like growth factor-binding protein 2 antibody; mIGFBP-2 antibody; IBP2 antibody; IGF-BP53 antibody; IBP-2 antibody; Igfbp-2 antibody; AI255832 antibody; mIGFBP-2 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
Produced in rabbits immunized with purified, recombinant Cynomolgus IGFBP-2 / IGFBP2 (rh IGFBP-2 / IGFBP2; F6YKM5; Met1-Gln324). IGFBP-2 / IGFBP2 specific IgG was purified by Cynomolgus IGFBP-2 / IGFBP2 affinity chromatography.
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Clonality
Polyclonal
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Formulation
0.2 μm filtered solution in PBS
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
WB: 2-10 μg/mL
ELISA: 0.1-0.2 μg/mL
This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Cynomolgus IGFBP-2 / IGFBP2. The detection limit for Cynomolgus IGFBP-2 / IGFBP2 is < 0.039 ng/well.
IHC-P: 1-4 μg/mL
IP: 1-4 μg/mg of lysate
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Validations
IGFBP-2 / IGFBP2 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunoprecipitation
IGFBP-2 / IGFBP2 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
Immunochemical staining of cynomolgus IGFBP2 in cynomolgus heart with rabbit polyclonal antibody (3 µg/mL, formalin-fixed paraffin embedded sections).
IGFBP-2 / IGFBP2 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
Immunochemical staining of cynomolgus IGFBP2 in cynomolgus kidney with rabbit polyclonal antibody (3 µg/mL, formalin-fixed paraffin embedded sections).
IGFBP-2 / IGFBP2 Antibody, Rabbit PAb, Antigen Affinity Purified, Western blot
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Background
IGFBP-2, also known as IGFBP2, is a insulin-like growth factor-binding protein (IGFBP). IGFBPs prolong the half-life of the IGFs , control bioavailability, activity, and distribution of insulin-like growth factor (IGF) through high-affinity IGFBP/IGF complexes. Six high-affinity IGF-binding proteins (IGFBP-1 to -6) have been identified. The six IGFBPs are structurally related but encoded by distinct genes. IGFBPs have a high affinity for IGFs. Some members of the IGFBP family have been consistently shown to inhibit IGF actions by preventing them from gaining access to the IGF receptors, while others potentiate IGF actions by facilitating the ligand-receptor interaction. IGFBP-2 is overexpressed in many malignancies and is often correlated with an increasingly malignant status of the tumor, pointing to a potential involvement of IGFBP-2 in tumorigenesis. It contains 1 IGFBP N-terminal domain and 1 thyroglobulin type-1 domain. It inhibits IGF-mediated growth and developmental rates.
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References
- Han VK, et al. (1996) The expression of insulin-like growth factor (IGF) and IGF-binding protein (IGFBP) genes in the human placenta and membranes: evidence for IGF-IGFBP interactions at the feto-maternal interface. J Clin Endocrinol Metab. 81(7): 2680-93.
- Binkert C, et al. (1989) Cloning, sequence analysis and expression of a cDNA encoding a novel insulin-like growth factor binding protein (IGFBP-2) . EMBO J. 8(9):2497-502.
- Wolf E, et al. (2000) Effects of IGFBP-2 overexpression in vitro and in vivo. Pediatr Nephrol. 14 (7):572-8.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"