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Product Name
Anti-IFNA5/IFNaG antibody
- Documents
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Description
Rabbit polyclonal to IFNA5/IFNaG
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Tested applications
ELISA, WB, IHC-P, IP
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Species reactivity
Rat IFNA5 / IFNaG
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Alternative names
Ifa5 antibody; IFNA5 antibody; Ifna5 antibody; RP11-380P16.5 antibody; RP23-139P14.29 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
Produced in rabbits immunized with purified, recombinant Rat IFNA5 / IFNaG (rh IFNA5 / IFNaG; XP_001076062.1; Met1-Glu189). IFNA5 / IFNaG specific IgG was purified by Rat IFNA5 / IFNaG affinity chromatography.
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Clonality
Polyclonal
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Formulation
0.2 μm filtered solution in PBS
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
WB: 10-20 μg/ml
ELISA:0.1-0.2 μg/mL
This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Rat IFNA5 / IFNaG. The detection limit for Rat IFNA5 / IFNaG is < 0.039 ng/well.
IHC-P: 0.1-2 μg/ml
IP: 4-6 μg/mg of lysate
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Validations
IFNA5 / IFNaG Antibody, Rabbit PAb, Antigen Affinity Purified, Western blot
IFNA5 / IFNaG Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
IFNA5 / IFNaG Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
IFNA5 / IFNaG Antibody, Rabbit PAb, Antigen Affinity Purified, Immunoprecipitation
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Background
Interferon, alpha 5 (IFNA5) belongs to the alpha/beta interferon family. IFNA5 is the only IFNA subtype detected in normal liver, while a mixture of subtypes is observed in the liver tissue of patients with chronic hepatitis C. Interferons are produced by macrophages, IFN-alpha have antiviral activities. Interferon stimulates the production of two enzymes: a protein kinase and an oligoadenylate synthetase. IFN-alpha, the first cytokine to be produced by recombinant DNA technology, has emerged as an important regulator of growth and differentiation, affecting cellular communication and signal transduction pathways as well as immunological control. Originally discovered as an antiviral substance, the efficacy of IFN-alpha in malignant, viral, immunological, angiogenic, inflammatory, and fibrotic diseases suggests a spectrum of interrelated pathophysiologies. IFN-alpha emerged as a prototypic tumor suppressor protein that represses the clinical tumorigenic phenotype in some malignancies capable of differentiation.
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References
- Lau JY, et al. (1993) Discrepancy between biochemical and virological responses to interferon-alpha in chronic hepatitis C. Lancet. 342(8881): 1208-9.
- Kessler DS, et al. (1990) Interferon-alpha regulates nuclear translocation and DNA-binding affinity of ISGF3, a multimeric transcriptional activator. Genes Dev. 4(10): 1753-65.
- Gutterman JU. Cytokine therapeutics: lessons from interferon alpha. Proc Natl Acad Sci U S A. 91(4): 1198-205.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"