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Product Name
Anti-Human IgG (9G6) Mouse antibody
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Description
Human IgG (9G6) Mouse monoclonal antibody
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Tested applications
WB
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Species reactivity
Human
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Isotype
Mouse IgG1
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Preparation
Antigen: Human IgG was isolated from human sera and purified by chromatography.
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Clonality
Monoclonal
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Formulation
Purified antibody in PBS containing 0.03% sodium azide.
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/500 - 1/2000
ELISA: 1/10000
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Validations
Western blot detection of human IgG using human IgG mouse mAb (1:1000 diluted).Predicted band size:50KDa.Observed band size:50KDa.
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Background
Monoclonal anti-human IgG is derived from the hybridoma1 produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. It is specific for the whole human IgG molecule, has no across action with human IgM molecule as determined by an ELISA. Reactivity is observed with all human IgG subclasses but not with the Fab fragment of human IgG, the antibody site is located in the terminal end of human IgG (part of the Fab fragment), the Fc portion has various important functions such as complement fixation, site for rheumatoid factor.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"