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Product Name
Anti-HLTF Rabbit antibody
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Description
HLTF Rabbit polyclonal antibody
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Tested applications
WB, IHC-P, ICC/IF
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Species reactivity
Human, Rat
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Alternative names
ZBU1; HLTF1; RNF80; HIP116; SNF2L3; HIP116A; SMARCA3 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthetic peptide of human HLTF
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Clonality
Polyclonal
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Formulation
Supplied in 50nM Tris-Glycine(pH 7.4), 0.15M Nacl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
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Storage instructions
Store at -20°C. Stable for 12 months from date of receipt.
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Applications
WB: 1/2000-1/10000
IHC: 1/20
ICC/IF: 1/20
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Validations
Immunocytochemistry of HLTF(green) in Hela cells using HLTF Rabbit pAb at dilution 1/50, and DAPI(blue)
Western blot detection of HLTF in Hela cell lysates using HLTF Rabbit pAb(1:1000 diluted).Predicted band size:114kDa.Observed band size:114kDa.
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Background
Swiss-Prot Acc.Q14527.Has both helicase and E3 ubiquitin ligase activities. Possesses intrinsic ATP-dependent nucleosome-remodeling activity; This activity may be required for transcriptional activation or repression of specific target promoters . These may include the SERPINE1 and HIV-1 promoters and the SV40 enhancer, to which this protein can bind directly. Plays a role in error-free postreplication repair (PRR) of damaged DNA and maintains genomic stability through acting as a ubiquitin ligase for 'Lys-63'-linked polyubiquitination of chromatin-bound PCNA.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"