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Product Name
Anti-Histone H3 (Phospho-Ser28) Rabbit antibody
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Description
Histone H3 (Phospho-Ser28) Rabbit polyclonal antibody
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Tested applications
WB, IHC-P, ICC/IF, FC
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Species reactivity
Human, Mouse, Rat
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Alternative names
H3F3; H3.3A antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthetic phosphopeptide corresponding to residues surrounding Ser28 of human Histone H3
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Clonality
Polyclonal
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Formulation
Supplied in 50nM Tris-Glycine(pH 7.4), 0.15M Nacl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
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Storage instructions
Store at -20°C. Stable for 12 months from date of receipt.
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Applications
WB: 1/1000
IHC: 1/50
ICC/IF: 1/50
FC: 1/20
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Validations
Western blot detection of Histone H3 (Phospho-Ser28) in 3T3,Hela cell lysates using Histone H3 (Phospho-Ser28) Rabbit pAb(1:1000 diluted).Predicted band size:15kDa.Observed band size:15kDa.
Immunohistochemistry of Histone H3 (Phospho-Ser28) in paraffin-embedded Human colon cancer tissue using Histone H3 (Phospho-Ser28) Rabbit pAb at dilution 1/50
Immunocytochemistry of Histone H3 (Phospho-Ser28)(green) in Hela cells using Histone H3 (Phospho-Ser28) Rabbit pAb at dilution 1/50, and DAPI(blue)
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Background
Swiss-Prot Acc.P68431.Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.MiscellaneousThis histone is only present in mammals and is enriched in acetylation of Lys-15 and dimethylation of Lys-10 (H3K9me2).CautionThe original paper reporting lysine deamination at Lys-5 by LOXL2 has been retracted due to inappropriate manipulation of figure data (PubMed:22483618, PubMed:27392148). However, this modification was confirmed in a subsequent publication (PubMed:27735137).
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