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Product Name
Anti-Hemopexin antibody
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Description
Mouse monoclonal to Hemopexin
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Tested applications
ELISA, IF, ICC/IF
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Species reactivity
Human HPX / Hemopexin
No cross-reactivity with Human cell lysate (293 cell line) in WB and ELISA. -
Alternative names
FLJ56652 antibody; Hemopexin antibody; Hemopexin antibody; Hpx antibody; HPX antibody; Hpxn antibody; HX antibody; Hx antibody; HX antibody; hx antibody; Hpxn antibody
- Immunogen
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Isotype
Mouse IgG1
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Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human HPX / Hemopexin (rhHPX; Met 1-His 462; NP_000604.1). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
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Clonality
Monoclonal
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Formulation
0.2 μm filtered solution in PBS with 5% trehalose
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
ELISA: 0.5-1 μg/mL
This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect HPX. The detection limit for HPX is approximately 0.625ng /well.
ICC/IF: 10-25 μg/mL
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Validations
Hemopexin / HPX Antibody, Mouse MAb, Immunofluorescence
Immunofluorescence staining of Human HPX in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-Human HPX monoclonal antibody (15 µg/ml) at 37℃ 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (green). Positive staining was localized to cytoplasm.
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Background
Binds heme and transports it to the liver for breakdown and iron recovery, after which the free hemopexin returns to the circulation.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"