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Product Name
Anti-Heme Oxygenase 1 Rabbit antibody
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Description
Heme Oxygenase 1 Rabbit polyclonal antibody
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Tested applications
WB, IHC-P, ICC/IF, FC, IP
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Species reactivity
Human, Mouse, Rat
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Alternative names
HO-1; HSP32; HMOX1D; bK286B10 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: Recombinant protein of mouse Heme Oxygenase 1
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Clonality
Polyclonal
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Formulation
Supplied in 50nM Tris-Glycine(pH 7.4), 0.15M Nacl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
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Storage instructions
Store at -20°C. Stable for 12 months from date of receipt.
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Applications
WB: 1/1000
IHC: 1/50
ICC/IF: 1/50
FC: 1/20
IP: 1/20
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Validations
Immunohistochemistry of Heme Oxygenase 1 in paraffin-embedded Human lung cancer tissue using Heme Oxygenase 1 Rabbit pAb at dilution 1/50
Western blot detection of Heme Oxygenase 1 in U251MG,K562,A549 cell lysates using Heme Oxygenase 1 Rabbit pAb(1:500 diluted).Predicted band size:33kDa.Observed band size:33kDa.
Western blot detection of Heme Oxygenase 1 in Mouse spleen lysates using Heme Oxygenase 1 Rabbit pAb(1:1000 diluted).Predicted band size:33KDa.Observed band size:33KDa.
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Background
Swiss-Prot Acc.P09601.Heme oxygenase cleaves the heme ring at the alpha methene bridge to form biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where senescent erythrocytes are sequestrated and destroyed. Exhibits cytoprotective effects since excess of free heme sensitizes cells to undergo apoptosis.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"