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Product Name
Anti-HBP1 antibody
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Description
Rabbit polyclonal antibody to HBP1
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Tested applications
WB, IHC-P
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Species reactivity
Human, Mouse, Rat, Chicken, Dog, Pig, Cow, Horse, Rabbit, Sheep
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Isotype
Rabbit IgG
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Preparation
This antigen of this antibody was klh conjugated synthetic peptide derived from human hbp1 421-514/514
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Clonality
Polyclonal
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Formulation
Liquid, 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
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Storage instructions
Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
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Applications
WB:1:500-2000
IHC-P:1:400-800
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Validations
Fig1: Protein: intestine(mouse) lysates at 40ug;; Primary: Anti-HBP1 at 1:200;; Secondary: HRP conjugated Goat Anti-Rabbit IgG(bs-0295G-HRP) at 1: 3000;; ECL excitated the fluorescence;; Predicted band size : 58kD; Observed band size : 58kD
Fig2: Sample:; placenta (mouse) Lysate at 40 ug; Uterus (Mouse) Lysate at 40 ug; bone (Mouse) Lysate at 40 ug; Primary: Anti-HBP1 at 1/300 dilution; Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution; Predicted band size: 58 kD; Observed band size: 63 kD
Fig3: Paraformaldehyde-fixed, paraffin embedded (Mouse intestine); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (HBP1) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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