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Product Name
Anti-HAGH antibody
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Description
Rabbit polyclonal to HAGH
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Tested applications
ELISA, WB, IHC-P, IP
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Species reactivity
Human HAGH / GLO2 / Glyoxalase II
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Alternative names
GLO2 antibody; Rsp29 antibody; BC019817 antibody; Glo2 antibody; Glo-2 antibody; GLX2 antibody; GLXII antibody; Glyoxalase II antibody; Glyoxalase II antibody; HAGH antibody; HAGH antibody; HAGH1 antibody; GLO2 antibody; GLX2 antibody; GLXII antibody; HAGH1 antibody; Glo2 antibody; Glo-2 antibody; Rsp29 antibody; BC019817 antibody
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Isotype
Rabbit IgG
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Preparation
Produced in rabbits immunized with purified, recombinant Human HAGH / GLO2 / Glyoxalase II (rh HAGH / GLO2 / Glyoxalase II; Q16775-2; Met 1-Asp 260). HAGH / GLO2 / Glyoxalase II specific IgG was purified by Human HAGH / GLO2 / Glyoxalase II affinity chromatography.
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Clonality
Polyclonal
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Formulation
0.2 μm filtered solution in PBS with 5% trehalose
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
WB: 1-10 μg/mL
ELISA: 0.1-0.2 μg/mL
This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human HAGH. The detection limit for Human HAGH is approximately 0.00245 ng/well.
IHC-P: 0.1-2 μg/mL
IP: 1-4 μg/mg of lysate
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Validations
HAGH / GLO2 / Glyoxalase II Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
Immunochemical staining of human HAGH in human kidney with rabbit polyclonal antibody (1 µg/mL, formalin-fixed paraffin embedded sections).
HAGH / GLO2 / Glyoxalase II Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
Immunochemical staining of human HAGH in human liver with rabbit polyclonal antibody (1 µg/mL, formalin-fixed paraffin embedded sections).
HAGH / GLO2 / Glyoxalase II Antibody, Rabbit PAb, Antigen Affinity Purified, Western blot
HAGH / GLO2 / Glyoxalase II Antibody, Rabbit PAb, Antigen Affinity Purified
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References
- Mulley JC, et al. (1987). New regional localisations for HAGH and PGP on human chromosome 16. Hum Genet 74 (4): 423-4.
- Rulli A, et al. (2001). Expression of glyoxalase I and II in normal and breast cancer tissues. Breast Cancer Res. Treat. 66 (1): 67-72.
- Cordell PA, et al. (2004). The Human hydroxyacylglutathione hydrolase (HAGH) gene encodes both cytosolic and mitochondrial forms of glyoxalase II. J Biol Chem. 279 (27): 28653-61.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"