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Product Name
Anti-HA antibody
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Description
Mouse monoclonal to HA
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Tested applications
ELISA, WB, FCM
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Species reactivity
H1N1 (A/California/04/2009) HA; H1N1 (A/California/07/2009) HA
Has cross-reactivity in ELISA with H1N2 (A/swine/Guangxi/13/2006) HA; H1N3 (A/duck/NZL/160/1976) HA; H5N1 (A/Anhui/1/2005) HA; H5N1 (A/Viet nam/1194/2004) HA; H5N1 (A/Indonesia/5/2005) HA; H5N1 (A/turkey/Turkey/1/2005) HA; H5N1 (A/bar-headed goose/Qinghai/14/2008) HA
No cross-reactivity in WB and ELISA with H1N1 (A/Brisbane/59/2007) HA; H1N1 (A/BrevigMission/1/1918) HA; H1N1 (A/Solomon Islands/3/2006) HA; H1N1 (A/Ohio/UR06-0091/2007) HA; H1N1 (A/New Caledonia/20/1999) HA; H1N1 (A/Puerto Rico/8/1934) HA; H1N1 (A/WSN/1933) HA; H3N2 (A/Brisbane/10/2007) HA; Influenza B (B/Florida/4/2006) HA; Human cell lysate (293 cell line) -
Alternative names
ha antibody; ihj antibody; sph antibody; nmf4 antibody; Spna1 antibody; Spna-1 antibody; AF093576 antibody; AI451697 antibody; CTLO antibody; HAMSV antibody; HRAS1 antibody; RASH1 antibody; p21ras antibody; C-H-RAS antibody; H-RASIDX antibody; C-BAS/HAS antibody; C-HA-RAS1 antibody
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Isotype
Mouse IgG2b
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Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Influenza A virus H1N1 hemagglutinin (HA) extracellular domain. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
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Clonality
Monoclonal
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Formulation
0.2 μm filtered solution in PBS with 5% trehalose
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
WB: 1-2 μg/mL
ELISA: 0.5-1 μg/mL
This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect H1N1 HA. The detection limit for H1N1 HA is approximately 10 ng/well.
FCM: 0.5-2 μg/Test
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Validations
Influenza A H1N1 (Swine Flu 2009) Hemagglutinin / HA Antibody, Mouse MAb, Flow cytometric
High Five cells were infected by Bac-HA, cells were collected at 48 hours postinfection, and the cell surface expression of HA were measured by flow cytometry. 106 Cells were stained with 1 μg Purified Mouse Anti-H1N1-HA (11055-MM08) antibody for 20 min on ice. Cells were washed twice and incubated with 1 μg of a FITC Goat Anti-Mouse Ig secondary antibody for 20 min on ice. Cells were washed twice and analyzed by flow cytometry.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"