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Product Name
Anti-HA antibody
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Description
Mouse monoclonal to HA
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Tested applications
ELISA, WB, FCM, IF, ICC/IF
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Species reactivity
Recognize N-terminal and C-terminal HA Tag in fusion proteins.
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Alternative names
ha antibody; ihj antibody; sph antibody; nmf4 antibody; Spna1 antibody; Spna-1 antibody; AF093576 antibody; AI451697 antibody; CTLO antibody; HAMSV antibody; HRAS1 antibody; RASH1 antibody; p21ras antibody; C-H-RAS antibody; H-RASIDX antibody; C-BAS/HAS antibody; C-HA-RAS1 antibody
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Isotype
Mouse IgG1
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Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, a synthetic peptide corresponding to the HA-tag sequence. The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
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Clonality
Monoclonal
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Formulation
0.2 μm filtered solution in PBS with 5% trehalose
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
WB: 1/2000-1/5000
ELISA: 1/2000-1/5000
This antibody can be used at 1/2000-1/5000 dilution with the appropriate secondary reagents to detect HA-tagged protein (amino-terminus HA tag or carboxy-terminus HA tag).
FCM: 1/50-1/200
ICC/IF: 1/200-1/500
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"