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Product Name
Anti-GTF2H1 (3E8) Mouse antibody
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Description
GTF2H1 (3E8) Mouse monoclonal antibody
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Tested applications
WB, IP, ICC/IF
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Species reactivity
Human
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Alternative names
Basic transcription factor 2 62 kDa subunit;Basic transcription factor 62 kDa subunit; BTF 2;BTF2;BTF2 p62;General transcription factor IIH polypeptide 1;General transcription factor IIH subunit 1;General transcription factor IIH antibody; polypeptide 1 antibody; 62kDa;gtf antibody
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Isotype
Mouse IgG1
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Preparation
Antigen: Purified recombinant human GTF2H1protein fragments expressed in E.coli.
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Clonality
Monoclonal
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Formulation
Purified mouse monoclonal antibody in buffer containing 0.1M Tris-Glycine(pH 7.4 150 mM NaCl)with 0.02% sodium azide 0.1mg/mlBSA and 50% glycerol.
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/300
;ICC: 1/50
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Validations
Immunoprecipitation analysis of Hela cell lysates using GTF2H1 mouse mAb.
Western blot detection of GTF2H1 in Hela,Hela NE and Jurkat cell lysates using GTF2H1 mouse mAb (1:300 diluted).Predicted band size: 62KDa.Observed band size: 62KDa.
Immunocytochemistry staining of HeLa cells fixed with 4% Paraformaldehyde and using GTF2H1 mouse mAb (dilution 1:50).
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Background
Swiss-Prot Acc.P32780.Component of the core-TFIIH basal transcription factor involved in nucleotide excision repair (NER) of DNA and, when complexed to CAK, in RNA transcription by RNA polymerase II.
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