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Product Name
Anti-Green fluorescent protein / GFP antibody
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Description
Mouse monoclonal to Green fluorescent protein / GFP
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Tested applications
ELISA, IF, IP, ICC/IF
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Species reactivity
Aequorea victoria GFP
No cross-reactivity in ELISA with E.coli cell lysate - Immunogen
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Isotype
Mouse IgG1
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Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Aequorea victoria GFP (AAB65663; Ser 2-Lys 238). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
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Clonality
Monoclonal
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Formulation
0.2 μm filtered solution in PBS with 5% trehalose
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
ELISA: 0.5-1 μg/mL
This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Aequorea victoria GFP. The detection limit for Aequorea victoria GFP is approximately 0.16 ng/well.
ICC/IF: 10-25 μg/mL
IP: 1-4 μg/mg of lysate
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Validations
GFP Antibody, Mouse Mab, Immunofluorescence
Immunofluorescence staining of GFP protein in CHO cells, transfected with GFP. Cells (left: GFP, middle: antibody, right: merge) were fixed with 4% PFA, blocked with 10% serum, and incubated with Mouse anti-GFP monoclonal antibody (15 µg/ml) at 37℃ 1 hour. Then cells were stained with the Alexa Fluor® 594-conjugated Goat Anti-mouse IgG secondary antibody (red) and counterstained with DAPI (blue).
GFP Antibody, Mouse MAb, Immunoprecipitation
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Background
The green fluorescent protein (GFP) is a protein that exhibit bright green fluorescence when exposed to blue light. GFPSparkTM is an improved variant of the green fluorescent protein GFP. It possesses bright green fluorescence (excitation/ emission max = 487 / 508 nm) that is visible earlier than fluorescence of other green fluorescent proteins. GFPSparkTM is mainly intended for applications where fast appearance of bright fluorescence is crucial. Its amazing ability to generate a highly visible, efficiently emitting internal fluorophore is both intrinsically fascinating and tremendously valuable. It is specially recommended for cell and organelle labeling and tracking the promoter activity.
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References
- Evdokimov et al. (2006). EMBO Rep, 7 (10):1006–1012 / pmid: 16936637.
- Haas et al. (1996). Curr Biol, 6 (3): 315–324 / pmid: 8805248.
- Kremers et al. (2006). Biochemistry, 45 (21): 6570–6580 / pmid: 16716067.
- Li et al. (1998). J Biol Chem, 273 (52): 34970–34975 /pmid: 9857028.
- Reid and Flynn (1997). Biochemistry, 36 (22): 6786–6791 / pmid: 9184161.
- Shagin et al. (2004). Curr Biol, 21 (5): 841–850 / pmid: 14963095.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"