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Anti-GRAMD1A antibody

Cat.#: 176650

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Product Information

  • Product Name
    Anti-GRAMD1A antibody
  • Documents
  • Description
    Mouse monoclonal antibody to GRAMD1A
  • Tested applications
    WB, ICC, IHC-P
  • Species reactivity
    Human, Mouse
  • Alternative names
    KIAA1533 antibody
  • Isotype
    IgM
  • Preparation
    This antigen of this antibody was recombinant protein
  • Clonality
    Monoclonal
  • Formulation
    Liquid, 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide
  • Storage instructions
    Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
  • Applications

    WB: 1:1,000-1:2,000

    ICC: 1:50-1:200

    IHC-P: 1:50-1:200

  • Validations

    Fig1: Western blot analysis of GRAMD1A on NIH/3T3 (1) and SHG-44 (2) cells lysates using anti-GRAMD1A antibody at 1/1,000 dilution.

    Fig1: Western blot analysis of GRAMD1A on NIH/3T3 (1) and SHG-44 (2) cells lysates using anti-GRAMD1A antibody at 1/1,000 dilution.

    Fig2: ICC staining GRAMD1A in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    Fig2: ICC staining GRAMD1A in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    Fig3: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-GRAMD1A antibody. Counter stained with hematoxylin.

    Fig3: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-GRAMD1A antibody. Counter stained with hematoxylin.

    Fig4: Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue using anti-GRAMD1A antibody. Counter stained with hematoxylin.

    Fig4: Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue using anti-GRAMD1A antibody. Counter stained with hematoxylin.

    Fig5: Immunohistochemical analysis of paraffin-embedded human glioma tissue using anti-GRAMD1A antibody. Counter stained with hematoxylin.

    Fig5: Immunohistochemical analysis of paraffin-embedded human glioma tissue using anti-GRAMD1A antibody. Counter stained with hematoxylin.

    Fig6: Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-GRAMD1A antibody. Counter stained with hematoxylin.

    Fig6: Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-GRAMD1A antibody. Counter stained with hematoxylin.

    Fig7: Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue using anti-GRAMD1A antibody. Counter stained with hematoxylin.

    Fig7: Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue using anti-GRAMD1A antibody. Counter stained with hematoxylin.

    Fig8: Immunohistochemical analysis of paraffin-embedded human stamoch tissue using anti-GRAMD1A antibody. Counter stained with hematoxylin.

    Fig8: Immunohistochemical analysis of paraffin-embedded human stamoch tissue using anti-GRAMD1A antibody. Counter stained with hematoxylin.

    Fig9: Immunohistochemical analysis of paraffin-embedded human gastric adenocarcinoma tissue using anti-GRAMD1A antibody. Counter stained with hematoxylin.

    Fig9: Immunohistochemical analysis of paraffin-embedded human gastric adenocarcinoma tissue using anti-GRAMD1A antibody. Counter stained with hematoxylin.

  • Background
  • References
    • Fu B et al. GRAM domain-containing protein 1A (GRAMD1A) promotes the expansion of hepatocellular carcinoma stem cell and hepatocellular carcinoma growth through STAT5. Sci Rep 6:31963 (2016).
    • Song X et al. Production and characterization of a monoclonal antibody against GRAM domain-containing protein 1A. Monoclon Antib Immunodiagn Immunother 33(4):246-53 (2014).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"