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Product Name
Anti-GRAMD1A antibody
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Description
Mouse monoclonal antibody to GRAMD1A
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Tested applications
WB, ICC, IHC-P
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Species reactivity
Human, Mouse
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Alternative names
KIAA1533 antibody
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Isotype
Mouse IgM
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Preparation
This antigen of this antibody was recombinant protein
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Clonality
Monoclonal
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Formulation
Liquid, 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide
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Storage instructions
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
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Applications
WB: 1:1,000-1:2,000
ICC: 1:50-1:200
IHC-P: 1:50-1:200
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Validations
Fig1: Western blot analysis of GRAMD1A on NIH/3T3 (1) and SHG-44 (2) cells lysates using anti-GRAMD1A antibody at 1/1,000 dilution.
Fig2: ICC staining GRAMD1A in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-GRAMD1A antibody. Counter stained with hematoxylin.
Fig4: Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue using anti-GRAMD1A antibody. Counter stained with hematoxylin.
Fig5: Immunohistochemical analysis of paraffin-embedded human glioma tissue using anti-GRAMD1A antibody. Counter stained with hematoxylin.
Fig6: Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-GRAMD1A antibody. Counter stained with hematoxylin.
Fig7: Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue using anti-GRAMD1A antibody. Counter stained with hematoxylin.
Fig8: Immunohistochemical analysis of paraffin-embedded human stamoch tissue using anti-GRAMD1A antibody. Counter stained with hematoxylin.
Fig9: Immunohistochemical analysis of paraffin-embedded human gastric adenocarcinoma tissue using anti-GRAMD1A antibody. Counter stained with hematoxylin.
- Background
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References
- Fu B et al. GRAM domain-containing protein 1A (GRAMD1A) promotes the expansion of hepatocellular carcinoma stem cell and hepatocellular carcinoma growth through STAT5. Sci Rep 6:31963 (2016).
- Song X et al. Production and characterization of a monoclonal antibody against GRAM domain-containing protein 1A. Monoclon Antib Immunodiagn Immunother 33(4):246-53 (2014).
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