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Product Name
Anti-GPX5 antibody
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Description
Mouse monoclonal antibody to GPX5
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Tested applications
WB, IHC-P, ICC
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Species reactivity
Human, Mouse
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Alternative names
EGLP antibody; GPx-5 antibody; GSHPx-5 antibody; HEL-S-75p antibody
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Isotype
Mouse IgG1
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Preparation
This antigen of this antibody was peptide
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Clonality
Monoclonal
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Formulation
Liquid, 1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
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Storage instructions
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
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Applications
WB: 1:500-1:2,000
IHC-P: 1:50-1:200
ICC: 1:50-1:200
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Validations
Fig1: Western blot analysis of GPX5 on human sperm tissue lysate using anti-GPX5 antibody at 1/5,000 dilution.
Fig2: Western blot analysis of GPX5 on mouse testis tissue lysate using anti-GPX5 antibody at 1/500 dilution.
Fig3: ICC staining GPX5 (green) in 293T cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: ICC staining GPX5 (green) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig5: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-GPX5 antibody. Counter stained with hematoxylin.
Fig6: Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-GPX5 antibody. Counter stained with hematoxylin.
Fig7: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-GPX5 antibody. Counter stained with hematoxylin.
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References
- Maremanda KP et al. Role of Zinc Supplementation in Testicular and Epididymal Damages in Diabetic Rat: Involvement of Nrf2, SOD1, and GPX5. Biol Trace Elem Res 173(2):452-64 (2016).
- Vilagran I et al. Triosephosphate isomerase (TPI) and epididymal secretory glutathione peroxidase (GPX5) are markers for boar sperm quality.Anim Reprod Sci 165:22-30 (2016).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"