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Product Name
Anti-Glypican 3/GPC3/OCI-5 antibody
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Description
Rabbit monoclonal to Glypican 3/GPC3/OCI-5
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Tested applications
FCM
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Species reactivity
Human Glypican 3 / GPC3 / OCI-5
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Alternative names
AC002420.1 antibody; DGSX antibody; Glypican 3 antibody; Glypican 3 antibody; GPC3 antibody; GPC3 antibody; GTR2-2 antibody; MXR7 antibody; OCI-5 antibody; OCI-5 antibody; RP23-33P21.1 antibody; SDYS antibody; SGB antibody; SGBS antibody; SGBS1 antibody; SGB antibody; DGSX antibody; MXR7 antibody; SDYS antibody; SGBS antibody; OCI-5 antibody; SGBS1 antibody; GTR2-2 antibody; OCI-5 antibody
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained from a rabbit immunized with a synthetic peptide corresponding to the center region of the Human Glypican 3 / GPC3 / OCI-5, and purified by antigen affinity chromatography.
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Clonality
Monoclonal
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Formulation
Aqueous solution containing 0.5% BSA and 0.09% sodium azide
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Storage instructions
This antibody is stable for 12 months from date of receipt when stored at 2℃-8℃. Protected from prolonged exposure to light. Do not freeze !
Sodium azide is toxic to cells and should be disposed of properly. Flush with large volumes of water during disposal. -
Applications
FCM
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Validations
Glypican 3 / GPC3 / OCI-5 Antibody, Rabbit MAb, Flow Cytometry
Flow cytometric analysis of Human GPC3 expression on HepG2 cells. Cells were stained with PE-conjugated anti-Human GPC3. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.
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Background
Glypican-3, also known as Intestinal protein OCI-5, GPC3, and OCI5, is a member of the glypican family. It belongs to the glypican family and is highly expressed in lung, liver, and kidney. It is a heparan sulfate proteoglycan, which is overexpressed in various neoplasms such as hepatocellular carcinoma, malignant melanoma, and testicular yolk sac tumor, and plays an important role in cell growth and differentiation. GPC3 function is tissue dependent. In some tissues, GPC3 acts as a tumor suppressor gene, whereas in others, it acts as an oncofetal protein. Studies have shown that GPC3 is a reliable marker for hepatocellular carcinoma. The sensitivity and specificity exceeds both alpha-fetoprotein and hepatocyte-paraffin1. GPC3 immunohistochemistry can aid in the differentiation of testicular germ cell tumors, being expressed in all yolk sac tumors but not in seminomas. GPC3 expression has also been identified in some squamous cell carcinomas of the lung and clear cell carcinomas of the ovary. The role of GPC3 in melanomas is still controversial. Thus, Glypican-3 is currently regarded as a tumor marker and potential target for immunotherapy.
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References
- Kandil DH, et al. (2009) Glypican-3: a novel diagnostic marker for hepatocellular carcinoma and more. Adv Anat Pathol. 16(2): 125-9.
- Maeda D, et al. (2009) Glypican-3 expression in clear cell adenocarcinoma of the ovary. Mod Pathol. 22(6): 824-32.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"