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Product Name
Anti-GGT1 antibody
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Description
Rabbit polyclonal to GGT1
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Tested applications
ELISA, IHC-P
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Species reactivity
Rat GGT1
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Alternative names
GGT antibody; GTG antibody; CD224 antibody; GGT1 antibody; D22S672 antibody; D22S732 antibody; GGT antibody; dwg antibody; Ggtp antibody; CD224 antibody; GGT1 antibody; GGT-1 antibody; CD224 antibody; CD224 antibody; D22S672 antibody; D22S732 antibody; dwg antibody; gamma-glutamyl transpeptidase antibody; gamma-glutamyl transpeptidase antibody; gamma-glutamyltranspeptidase 1 antibody; gamma-glutamyltranspeptidase 1 antibody; GGT antibody; GGT antibody; GGT 1 antibody; GGT 1 antibody; Ggtp antibody; glutamyl transpeptidase antibody; glutathione hydrolase 1 antibody; glutathione hydrolase 1 antibody; GTG antibody; leukotriene-C4 hydrolase antibody; leukotriene-C4 hydrolase antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
Produced in rabbits immunized with purified, recombinant Rat GGT1 (rh GGT1; P07314; Pro27-Tyr568). GGT1 specific IgG was purified by Rat GGT1 affinity chromatography.
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Clonality
Polyclonal
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Formulation
0.2 μm filtered solution in PBS
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
WB: 10-20 μg/ml
ELISA:0.1-0.2 μg/mL
This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Rat GGT1. The detection limit for Rat GGT1 is < 0.039 ng/well.
IHC-P: 0.05-1 μg/ml
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Validations
GGT1 Antibody, Rabbit PAb, Antigen Affinity Purified, Western blot
GGT1 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
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Background
GGT1 belongs to the gamma-glutamyltransferase protein family. Many members of this family have not yet been fully characterized and some of which may represent pseudogenes. GGT1 is composed of a heavy chain and a light chain. It catalyzes the transfer of the glutamyl moiety of glutathione to a variety of amino acids and dipeptide acceptors. GGT1 also initiates extracellular glutathione (GSH) breakdown, provides cells with a local cysteine supply and contributes to maintain intracelular GSH level. As part of the cell antioxidant defense mechanism, GGT1 can be detected in fetal and adult kidney and liver, adult pancreas, stomach, intestine, placenta and lung. Defects in GGT1 can cause glutathionuria which is known as an autosomal recessive disease.
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References
- Bulle F, et al. (1987) Assignment of the human gamma-glutamyl transferase gene to the long arm of chromosome 22. Hum Genet. 76(3):283-6.
- Tate SS, et al. (1988) Renal gamma-glutamyl transpeptidases: structural and immunological studies. Arch Biochem Biophys. 262(2):397-408.
- Tate SS, et al. (1988) In vitro translation and processing of human hepatoma cell (Hep G2) gamma-glutamyl transpeptidase. Biochem Biophys Res Commun. 154(3):1167-73.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"