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Product Name
Anti-GGCT antibody
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Description
Rabbit polyclonal to GGCT
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Tested applications
ELISA, WB, IHC-P, IP
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Species reactivity
Human GGCT
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Alternative names
GGC antibody; GCTG antibody; CRF21 antibody; C7orf24 antibody; Ggc antibody; Gctg antibody; AA673177 antibody; A030007L17Rik antibody; A030007L17Rik antibody; AA673177 antibody; C7orf24 antibody; CRF21 antibody; cytochrome c-releasing factor 21 antibody; gamma -glutamyl cyclotransferase antibody; gamma-glutamyl cyclotransferase antibody; gamma-glutamylcyclotransferase antibody; gamma-glutamylcyclotransferase antibody; Gctg antibody; GCTG antibody; Ggc antibody; GGC antibody; Ggct antibody; GGCT antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
Produced in rabbits immunized with purified, recombinant Human GGCT (rh GGCT; O75223-1; Met1-Leu188). GGCT specific IgG was purified by Human GGCT affinity chromatography.
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Clonality
Polyclonal
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Formulation
0.2 μm filtered solution in PBS
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
WB: 1-10 μg/mL
ELISA: 0.1-0.2 μg/mL
This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human GGCT. The detection limit for Human GGCT is < 0.039 ng/well.
IHC-P: 0.1-1 μg/mL
IP: 1-4 μg/mg of lysate
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Validations
GGCT Antibody, Rabbit PAb, Antigen Affinity Purified, Immunoprecipitation
GGCT Antibody, Rabbit PAb, Antigen Affinity Purified
Immunochemical staining of human GGCT in human hepatoma with rabbit polyclonal antibody (0.1 µg/mL, formalin-fixed paraffin embedded sections).
GGCT Antibody, Rabbit PAb, Antigen Affinity Purified, Western blot
GGCT Antibody, Rabbit PAb, Antigen Affinity Purified
Immunochemical staining of human GGCT in human corpus uteri with rabbit polyclonal antibody (0.1 µg/mL, formalin-fixed paraffin embedded sections).
GGCT Antibody, Rabbit PAb, Antigen Affinity Purified
Immunochemical staining of human GGCT in human lung cancer with rabbit polyclonal antibody (0.1 µg/mL, formalin-fixed paraffin embedded sections).
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Background
GGCT belongs to the gamma-glutamylcyclotransferase family. It catalyzes the formation of 5-oxoproline from gamma-glutamyl dipeptides, the penultimate step in glutathione catabolism. GGCT may play a significant role in glutathione homeostasis. GGCT also induces release of cytochrome c from mitochondria with resultant induction of apoptosis. Pseudogenes of GGCT gene are located on the long arm of chromosome 5 and the short arm of chromosomes 2 and 20. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
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References
- Wagner SA. et al., 2011, Mol Cell Proteomics. 10 (10): M111.013284.
- Kim W. et al., 2011, Mol Cell. 44 (2): 325-40.
- Amano T. et al., 2012, J Histochem Cytochem. 60 (1): 76-86.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"