-
Product Name
Anti-GAPDH antibody
- Documents
-
Description
Rabbit polyclonal to GAPDH
-
Tested applications
IHC-P
-
Species reactivity
Human GAPDH
-
Alternative names
G3PD antibody; GAPD antibody; HEL-S-162eP antibody; Gapd antibody; CDABP0047 antibody; G3PD antibody; Gapd antibody; GAPD antibody; MGC102544 antibody; MGC102546 antibody; MGC103191 antibody; MGC88685 antibody; MGC103190 antibody; MGC105239 antibody
-
Isotype
Rabbit IgG
-
Preparation
Produced in rabbits immunized with A synthetic peptide corresponding to the center region of the human GAPDH, and purified by antigen affinity chromatography.
-
Clonality
Polyclonal
-
Formulation
0.2 μm filtered solution in PBS
-
Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
IHC-P: 0.1-2 μg/mL
-
Validations
GAPDH Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
Immunochemical staining of human GAPDH in human hepatitis with rabbit polyclonal antibody (0.5 µg/mL, formalin-fixed paraffin embedded sections).
GAPDH Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
Immunochemical staining of human GAPDH in human renal carcinoma with rabbit polyclonal antibody (0.5 µg/mL, formalin-fixed paraffin embedded sections).
-
Background
Glyceraldehyde 3-phosphate dehydrogenase (GAPDH or G3PDH) is an enzyme of about 37kDa that is consisdered as a cellular enzyme involved in glycolysis. It catelyzes the sixth step of glycolysis. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a pleiotropic enzyme that is overexpressed in apoptosis and in several human chronic pathologies. Its role as a mediator for cell death has also been highlighted. A recent report suggests that GAPDH may be genetically associated with late-onset of Alzheimer's disease. Besides, deprenyl, which has originally been used as a monoamine oxidase inhibitor for Parkinson's disease, binds to GAPDH and displays neuroprotective actions.
-
References
- Hara MR, et al. (2006) Neuroprotection by pharmacologic blockade of the GAPDH death cascade. PNA. 103 (10): 3887-9.
- Hara MR, et al. (2006) GAPDH as a sensor of NO stress.Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease. 1762 (5): 502-9.
- Tarze A, et al. (2007) GAPDH, a novel regulator of the pro-apoptotic mitochondrial membrane permeabilizationGAPDH and apoptosis. Oncogene. 26: 2606-20.
- Yi MK, et al. (2000) Functional Significance of the Interaction of Hepatitis A Virus RNA with Glyceraldehyde 3-Phosphate Dehydrogenase (GAPDH): Opposing Effects of GAPDH and Polypyrimidine Tract Binding Protein on Internal Ribosome Entry Site Function. Journal of Virology. 74 (14) : 6459-68.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"