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Product Name
Anti-G3BP Rabbit antibody
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Description
G3BP Rabbit monoclonal antibody
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Tested applications
WB, ICC/IF, FC, IP
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Species reactivity
Human
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Alternative names
G3BP; HDH-VIII antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthetic peptide of human G3BP
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Clonality
Monoclonal
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Formulation
Supplied in 50nM Tris-Glycine(pH 7.4), 0.15M Nacl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
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Storage instructions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/2000-1/10000
ICC/IF: 1/50-1/200
FC: 1/20
IP: 1/20
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Validations
Western blot detection of G3BP in K562,C6,Hela cell lysates using G3BP Rabbit mAb(1:1000 diluted).Predicted band size:52kDa.Observed band size:68kDa.
Immunocytochemistry of G3BP(green) in Hela cells using G3BP Rabbit mAb at dilution 1/200, and DAPI(blue)
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Background
Swiss-Prot Acc.Q13283.ATP- and magnesium-dependent helicase (PubMed:9889278). Unwinds preferentially partial DNA and RNA duplexes having a 17 bp annealed portion and either a hanging 3' tail or hanging tails at both 5'- and 3'-ends (PubMed:9889278). Unwinds DNA/DNA, RNA/DNA, and RNA/RNA substrates with comparable efficiency (PubMed:9889278). Acts unidirectionally by moving in the 5' to 3' direction along the bound single-stranded DNA (PubMed:9889278). Phosphorylation-dependent sequence-specific endoribonuclease in vitro (PubMed:11604510). Cleaves exclusively between cytosine and adenine and cleaves MYC mRNA preferentially at the 3'-UTR (PubMed:11604510). May be a regulated effector of stress granule assembly (PubMed:12642610, PubMed:20180778).
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"