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Product Name
Anti-FSTL1 antibody
- Documents
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Description
Rabbit polyclonal to FSTL1
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Tested applications
ELISA, WB, IHC-P
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Species reactivity
Mouse FSTL1
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Alternative names
Fstl antibody; TSC-36 antibody; AI316791 antibody; AW107808 antibody; FRP antibody; FSL1 antibody; MIR198 antibody; AI316791 antibody; AW107808 antibody; FLJ50214 antibody; FLJ52277 antibody; FRP antibody; FSL1 antibody; Fstl antibody; FSTL1 antibody; Fstl1 antibody; TSC-36 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
Produced in rabbits immunized with purified, recombinant Mouse FSTL1 (rh FSTL1; NP_032073.2; Met1-Ile306). FSTL1 specific IgG was purified by Mouse FSTL1 affinity chromatography.
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Clonality
Polyclonal
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Formulation
0.2 μm filtered solution in PBS
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
WB: 10-20 μg/ml
ELISA:0.1-0.2 μg/mL
This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Mouse FSTL1. The detection limit for Mouse FSTL1 is < 0.039 ng/well.
IHC-P: 0.1-1 μg/ml
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Validations
FSTL1 Antibody, Rabbit PAb, Antigen Affinity Purified, Western blot
FSTL1 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
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Background
Follistatin-related protein 1 (FSTL1) is an extracellular glycoprotein whose functional significance in physiological and pathological processes is incompletely understood. Recently, we have shown that FSTL1 acts as a muscle-derived secreted factor that is up-regulated by Akt activation and ischemic stress and that FSTL1 exerts favorable actions on the heart and vasculature. Here, we sought to identify the receptor that mediates the cellular actions of FSTL1. It contains an FS module, a follistatin-like sequence containing 10 conserved cysteine residues. FSTL1 is thought to be an autoantigen associated with rheumatoid arthritis. DIP2A functions as a novel receptor that mediates the cardiovascular protective effects of FSTL1. Experiment results have provided in vivo and in vitro evidence to demonstrate that Fstl1 modulates lung development and alveolar maturation, in part, through BMP4 signaling.
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References
- Rosenberg MI, et al. (2006) MyoD inhibits Fstl1 and Utrn expression by inducing transcription of miR-206. J Cell Biol. 175(1): 77-85.
- Ouchi N, et al. (2010) DIP2A functions as a FSTL1 receptor. J Biol Chem. 285(10): 7127-34.
- Geng Y, et al. (2011) Follistatin-like 1 (Fstl1) is a bone morphogenetic protein (BMP) 4 signaling antagonist in controlling mouse lung development. Proc Natl Acad Sci U S A. 108(17): 7058-63.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"