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Product Name
Anti-FLAG tag antibody
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Description
Mouse monoclonal to FLAG tag
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Tested applications
WB, IP
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Species reactivity
Recognize N-terminal and C-terminal Flag Tag in fusion proteins.
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Isotype
Mouse IgG2b
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Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, the synthetic peptide corresponding to the DYKDDDDK-tag sequence (CDYKDDDDK). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
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Clonality
Monoclonal
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Formulation
0.2 μm filtered solution in PBS with 5% trehalose
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
WB: 4-50 μg/mL
IP: 1-4 μg/mg of lysate
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Validations
Anti-DYKDDDDK (FLAG® epitope Tag) Antibody, Mouse MAb, Immunoprecipitation
Anti-DYKDDDDK (FLAG® epitope Tag) Antibody, Mouse MAb, Western blot
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"