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Product Name
Anti-FEN1 antibody
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Description
Rabbit polyclonal to FEN1
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Tested applications
WB, IHC-P, IF, IP, ICC/IF
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Species reactivity
Human FEN1
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Alternative names
MF1 antibody; RAD2 antibody; FEN-1 antibody; FEN-1 antibody; AW538437 antibody
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Isotype
Rabbit IgG
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Preparation
Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the human FEN1, and purified by antigen affinity chromatography.
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Clonality
Polyclonal
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Formulation
0.2 μm filtered solution in PBS
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
WB: 2-10 μg/ml
IHC-P: 0.1-2 μg/mL
ICC/IF: 0.5-1.5 μg/mL
IP: 1-4 μg/mg of lysate
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Validations
FEN1 Antibody, Rabbit PAb, Antigen Affinity Purified, Western blot
FEN1 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
Immunochemical staining of human FEN1 in human tonsil with rabbit polyclonal antibody (0.5 µg/mL, formalin-fixed paraffin embedded sections).
FEN1 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
Immunochemical staining of human FEN1 in human testis with rabbit polyclonal antibody (0.5 µg/mL, formalin-fixed paraffin embedded sections).
FEN1 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunofluorescence
Immunofluorescence staining of FEN1 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human FEN1 polyclonal antibody (1 µg/ml) at 4℃ overnight. Then cells were stained with the Alexa Fluor®594-conjugated Goat Anti-rabbit IgG secondary antibody (red)Positive staining was localized to nucleus.
FEN1 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunoprecipitation
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"