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Product Name
Anti-FEN1 (7H8) Mouse antibody
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Description
FEN1 (7H8) Mouse monoclonal antibody
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Tested applications
WB, ICC/IF
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Species reactivity
Human, Mouse, Rat, Monkey, Hamster
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Alternative names
DNase IV;FEN-1;FEN1;FEN1_HUMAN;Flap endonuclease 1;Flap structure specific endonuclease 1;Flap structure-specific endonuclease 1;hFEN-1;hFEN1;Maturation factor 1;MF1;Rad2. antibody
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Isotype
Mouse IgG1
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Preparation
Antigen: Purified recombinant human FEN-1 protein fragments expressed in E.coli.
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Clonality
Monoclonal
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Formulation
Purified mouse monoclonal antibody in PBS(pH 7.4) containing with 0.02% sodium azide and 50% glycerol.
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/1000
;ICC: 1/400
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Validations
Western blot detection of FEN-1 in Hela,Jurkat,3T3,COS7,PC-12,C6,Raw264.7,Raji and CHO-K1 cell lysates using FEN-1 mouse mAb (1:1000 diluted).Predicted band size:45KDa.Observed band size:45KDa.
Immunocytochemistry staining of HeLa cells fixed with 4% Paraformaldehyde and using FEN-1 mouse mAb (dilution 1:400).
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Background
Swiss-Prot Acc.P39748.Acts as a genome stabilization factor that prevents flaps from equilibrating into structurs that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA, and exhibits RNase H activity. Also involved in replication and repair of rDNA and in repairing mitochondrial DNA.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"