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Product Name
Anti-FANCC antibody
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Description
Rabbit polyclonal to FANCC
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Tested applications
IHC-P, IF, ICC/IF
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Species reactivity
Human FANCC
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Alternative names
FA3 antibody; FAC antibody; FACC antibody; Facc antibody; mir-3074-1 antibody
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Isotype
Rabbit IgG
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Preparation
Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human FANCC, and purified by antigen affinity chromatography.
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Clonality
Polyclonal
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Formulation
0.2 μm filtered solution in PBS
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
IHC-P: 0.1-2 μg/mL
ICC/IF: 0.5-1.5 μg/mL
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Validations
FANCC Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
Immunochemical staining of human FANCC in human brain with rabbit polyclonal antibody (0.5 µg/mL, formalin-fixed paraffin embedded sections).
FANCC Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
Immunochemical staining of human FANCC in human pancreas with rabbit polyclonal antibody (0.5 µg/mL, formalin-fixed paraffin embedded sections).
FANCC Antibody, Rabbit PAb, Antigen Affinity Purified, Immunofluorescence
Immunofluorescence staining of FANCC in HeLa cells. Cells were fixed with 4% PFA,blocked with 10% serum. Then incubated with rabbit anti-human FANCC polyclonal antibody (1 µg/ml)(Figure A), incubated with rabbit anti-human FANCC polyclonal antibody and antigen (Figure B) at 4℃ overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm and nucleus.
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