-
Product Name
Anti-FAM83H antibody
- Documents
-
Description
Rabbit polyclonal antibody to FAM83H
-
Tested applications
WB, IHC-P
-
Species reactivity
Human, Mouse, Rat, Dog, Cow, Horse, Sheep
-
Alternative names
AI3 antibody; AI3A antibody
-
Isotype
Rabbit IgG
-
Preparation
This antigen of this antibody was klh conjugated synthetic peptide derived from human fam83h 1001-1179/1179
-
Clonality
Polyclonal
-
Formulation
Liquid, 0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
-
Storage instructions
Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
-
Applications
IHC-P:1:400-800
-
Validations
Fig1: Paraformaldehyde-fixed, paraffin embedded (Rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (FAM83H) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Fig2: Protein: HL-60(human) lysate at 40ug;; Primary: rabbit Anti-FAM83H at 1:300;; Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000;; Predicted band size: 127 kD; Observed band size: 127 kD
- Background
-
References
- Kuga T et. al. A novel mechanism of keratin cytoskeleton organization through casein kinase Ialpha and FAM83H in colorectal cancer. J. Cell Sci. 126:4721-4731(2013).
- Kim J.-W. et. al. FAM83H mutations in families with autosomal-dominant hypocalcified amelogenesis imperfecta. Am. J. Hum. Genet. 82:489-494(2008).
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"