-
Product Name
Anti-FABP6/ILBP/I-BABP antibody
- Documents
-
Description
Rabbit monoclonal to FABP6/ILBP/I-BABP
-
Tested applications
FCM, IF, ICC/IF
-
Species reactivity
Human FABP6 / ILBP / I-BABP
-
Alternative names
ILBP antibody; I-15P antibody; I-BAP antibody; ILBP3 antibody; ILLBP antibody; I-BABP antibody; I-BALB antibody; GT antibody; ILBP antibody; I-15P antibody; ILBP3 antibody; Illbp antibody; I-BABP antibody; Fabp6 antibody; FABP6 antibody; I-15P antibody; I-15P antibody; I-BABP antibody; I-BABP antibody; I-BALB antibody; I-BAP antibody; ILBP antibody; ILBP antibody; ILBP3 antibody; Illbp antibody; ILLBP antibody; RP23-26M1.3 antibody
- Immunogen
-
Isotype
Rabbit IgG
-
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human FABP6 / ILBP / I-BABP (rh FABP6 / ILBP / I-BABP; AAH22489.1; Met1-Ala128).
-
Clonality
Monoclonal
-
Formulation
0.2 μm filtered solution in PBS
-
Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
FCM: 0.5-2 μg/Test
ICC/IF: 10-25 μg/mL
-
Validations
FABP6 / ILBP / I-BABP Antibody, Rabbit MAb
Flow cytometric analysis of Human FABP6 expression on MCF-7 cells. The cells were treated according to manufacturer’s manual (BD Pharmingen™ Cat. No. 554714), stained with purified anti-Human FABP6, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.
FABP6 / ILBP / I-BABP Antibody, Rabbit MAb, Immunofluorescence
Immunofluorescence staining of Human FABP6 in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with rabbit anti-Human FABP6 monoclonal antibody (15 µg/ml) at 37℃ 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.
-
Background
Gastrotropin, also known as Fatty acid-binding protein 6, Ileal lipid-binding protein, ILBP, Intestinal 15 kDa protein, Intestinal bile acid-binding protein, I-BABP and FABP6, is a cytoplasm protein which belongs to the calycin superfamily and Fatty-acid binding protein (FABP) family. Isoform 2 of FABP6 is expressed in colorectal adenocarcinomas and their adjacent normal mucosa (at protein level). Isoform 1 of FABP6 is expressed in the jejunum, ileum, cecum and ascending colon intestine. Isoform 2 is expressed in the gallbladder, duodenum, jejunum, ileum, cecum, ascending, transverse and descending colon, sigmoid colon and rectum. FABP6 / I-BABP is a cancer-related protein that acts as an intracellular transporter of bile acid in the ileal epithelium. FABP6 / I-BABP may also play an important role in early carcinogenesis.
-
References
- Kurz M., et al., 2003, Proteins 50:312-328.
- Ohmachi,T. et al., 2006, Clin Cancer Res.12 (17):5090-5.
- Fang C., et al., 2007, Cancer Res. 67:9039-9046.
- Szczerbal,I. et al., 2007, Cytogenet Genome Res.118 (1):63-6.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"