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Anti-FABP-1 (6C6) Mouse antibody

Cat.#: 168279

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Special Price 441.3 USD

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Product Information

  • Product Name
    Anti-FABP-1 (6C6) Mouse antibody
  • Documents
  • Description
    FABP-1 (6C6) Mouse monoclonal antibody
  • Tested applications
    WB, ICC/IF
  • Species reactivity
    Human, Mouse, Rat, Monkey
  • Isotype
    Mouse IgG1
  • Preparation
    Antigen: Purified recombinant fragment of human GOT2 expressed in E. Coli.
  • Clonality
    Monoclonal
  • Formulation
    Ascitic fluid containing 0.03% sodium azide.
  • Storage instructions
    Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Applications

    WB: 1/500 - 1/2000

    ICC: 1/200 - 1/1000

    ELISA: 1/10000

  • Validations

    Western blot detection of GOT2 in MCF7,HCT116,Jurkat and Raji cell lysates using GOT2 mouse mAb (1:1000 diluted).Predicted band size:47KDa.Observed band size:47KDa.

    Western blot detection of GOT2 in MCF7,HCT116,Jurkat and Raji cell lysates using GOT2 mouse mAb (1:1000 diluted).Predicted band size:47KDa.Observed band size:47KDa.

    Immunofluorescence analysis of PC-3 (left) and SK-BR-3 (right) cells using anti-GOT2 mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.

    Immunofluorescence analysis of PC-3 (left) and SK-BR-3 (right) cells using anti-GOT2 mAb (green). Red: Actin filaments have been labeled with DY-554 phalloidin. Blue: DRAQ5 fluorescent DNA dye.

  • Background
    Swiss-Prot Acc.P00505.Glutamic-oxaloacetic transaminase is a pyridoxal phosphate-dependent enzyme which exists in cytoplasmic andinner-membrane mitochondrial forms, GOT1 and GOT2, respectively. GOT plays a role in amino acid metabolism and theurea and tricarboxylic acid cycles. The two enzymes are homodimeric and show close homology.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"