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Product Name
Anti-ERM/ETV5 (3H4) Mouse antibody
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Description
ERM/ETV5 (3H4) Mouse monoclonal antibody
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Tested applications
WB, ICC/IF
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Species reactivity
Human, Rat, Mouse
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Isotype
Mouse IgG1
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Preparation
Antigen: Purified recombinant fragment of human ETV5 expressed in E. Coli.
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Clonality
Monoclonal
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Formulation
Ascitic fluid containing 0.03% sodium azide.
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/500 - 1/2000
IF: 1/200- 1/500
ELISA: 1/10000
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Validations
Immunocytochemistry staining of HeLa cells fixed with 1% Paraformaldehyde and using anti-ERM/Etv5 mouse mAb (dilution 1:500).
Western blot detection of ERM/Etv5 in C6,3T3 and K562 cell lysates using ERM/Etv5 mouse mAb (1:500 diluted).Predicted band size:58KDa.Observed band size:58KDa.
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Background
Swiss-Prot Acc.P41161.ETS variant 5,transcription factor of the ETS family,divergent member of the winged helix-turn-helix superfamily,expressed in brain,placenta and other tissues involved in development. Tissue specificity: Ubiquitous. ERM is expressed in Sertoli cells and spermatogonial stem cells (SSCs). Studies on knockout mice have shown that it is required for SSC self-renewal but not differentiation, thus affected mice exhibit progressive germ cell depletion.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"