Anti-ERM/ETV5 (3H4) Mouse antibody

Cat.#: 168396

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Product Information

  • Product Name
    Anti-ERM/ETV5 (3H4) Mouse antibody
  • Documents
  • Description
    ERM/ETV5 (3H4) Mouse monoclonal antibody
  • Tested applications
    WB, ICC/IF
  • Species reactivity
    Human, Rat, Mouse
  • Isotype
    Mouse IgG1
  • Preparation
    Antigen: Purified recombinant fragment of human ETV5 expressed in E. Coli.
  • Clonality
    Monoclonal
  • Formulation
    Ascitic fluid containing 0.03% sodium azide.
  • Storage instructions
    Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Applications

    WB: 1/500 - 1/2000

    IF: 1/200- 1/500

    ELISA: 1/10000

  • Validations

    Immunocytochemistry staining of HeLa cells fixed with 1% Paraformaldehyde and using anti-ERM/Etv5 mouse mAb (dilution 1:500).

    Immunocytochemistry staining of HeLa cells fixed with 1% Paraformaldehyde and using anti-ERM/Etv5 mouse mAb (dilution 1:500).

    Western blot detection of ERM/Etv5 in C6,3T3 and K562 cell lysates using ERM/Etv5 mouse mAb (1:500 diluted).Predicted band size:58KDa.Observed band size:58KDa.

    Western blot detection of ERM/Etv5 in C6,3T3 and K562 cell lysates using ERM/Etv5 mouse mAb (1:500 diluted).Predicted band size:58KDa.Observed band size:58KDa.

  • Background
    Swiss-Prot Acc.P41161.ETS variant 5,transcription factor of the ETS family,divergent member of the winged helix-turn-helix superfamily,expressed in brain,placenta and other tissues involved in development. Tissue specificity: Ubiquitous. ERM is expressed in Sertoli cells and spermatogonial stem cells (SSCs). Studies on knockout mice have shown that it is required for SSC self-renewal but not differentiation, thus affected mice exhibit progressive germ cell depletion.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"