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Product Name
Anti-ERCC1 Rabbit antibody
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Description
ERCC1 Rabbit monoclonal antibody
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Tested applications
WB, ICC/IF, FC
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Species reactivity
Human, Mouse
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Alternative names
UV20; COFS4; RAD10 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthetic peptide of human ERCC1
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Clonality
Monoclonal
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Formulation
Supplied in 50nM Tris-Glycine(pH 7.4), 0.15M Nacl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
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Storage instructions
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/1000-1/5000
ICC/IF: 1/20-1/100
FC: 1/50
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Validations
Immunocytochemistry of ERCC1(green) in Hela cells using ERCC1 Rabbit mAb at dilution 1/200, and DAPI(blue)
Western blot detection of ERCC1 in K562,3T3,Hela cell lysates using ERCC1 Rabbit mAb(1:1000 diluted).Predicted band size:33kDa.Observed band size:39kDa.
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Background
Swiss-Prot Acc.P07992.Isoform 1: Non-catalytic component of a structure-specific DNA repair endonuclease responsible for the 5'-incision during DNA repair. Responsible, in conjunction with SLX4, for the first step in the repair of interstrand cross-links (ICL). Participates in the processing of anaphase bridge-generating DNA structures, which consist in incompletely processed DNA lesions arising during S or G2 phase, and can result in cytokinesis failure. Also required for homology-directed repair (HDR) of DNA double-strand breaks, in conjunction with SLX4.RegionsFeature keyPosition(s)Description
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"