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Product Name
Anti-ENTPD5 antibody
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Description
Rabbit monoclonal to ENTPD5
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Tested applications
WB, IHC-P, IP
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Species reactivity
Human ENTPD5
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Alternative names
PCPH antibody; CD39L4 antibody; NTPDase-5 antibody; Pcph antibody; Cd39l4 antibody; mNTPase antibody; AI196558 antibody; AI987697 antibody; NTPDase5 antibody; ER-UDPase antibody; NTPDase-5 antibody; AI196558 antibody; AI987697 antibody; CD39 antigen-like 4 antibody; CD39 antigen-like 4 antibody; CD39L4 antibody; Cd39l4 antibody; CD39-like 4 antibody; ER-UDPase antibody; ER-UDPase antibody; GDPase ENTPD5 antibody; GDPase ENTPD5 antibody; guanosine-diphosphatase ENTPD5 antibody; guanosine-diphosphatase ENTPD5 antibody; mNTPase antibody; NTPDase 5 antibody; NTPDase 5 antibody; NTPDase5 antibody; nucleoside diphosphatase antibody; nucleoside diphosphatase antibody; Pcph antibody; PCPH antibody; Pcph proto-oncogene protein antibody; proto-oncogene CPH antibody; UDPase ENTPD5 antibody; UDPase ENTPD5 antibody; uridine-diphosphatase ENTPD5 antibody; uridine-diphosphatase ENTPD5 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Human ENTPD5 (rh ENTPD5; O75356; Met1-His428).
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Clonality
Monoclonal
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Formulation
0.2 μm filtered solution in PBS
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
WB: 10-20 μg/ml
IHC-P: 1-10 μg/mL
IP: 4-6 μg/mg of lysate
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Validations
ENTPD5 Antibody, Rabbit MAb, Western blot
ENTPD5 Antibody, Rabbit MAb, Immunohistochemistry
Immunochemical staining of human ENTPD5 in human liver with rabbit monoclonal antibody (5 µg/mL, formalin-fixed paraffin embedded sections).
ENTPD5 Antibody, Rabbit MAb, Immunoprecipitation
ENTPD5 Antibody, Rabbit MAb, Immunohistochemistry
Immunochemical staining of human ENTPD5 in human kidney with rabbit monoclonal antibody (5 µg/mL, formalin-fixed paraffin embedded sections).
ENTPD5 Antibody, Rabbit MAb, Immunohistochemistry
Immunochemical staining of human ENTPD5 in human prostate with rabbit monoclonal antibody (5 µg/mL, formalin-fixed paraffin embedded sections).
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Background
Ectonucleoside triphosphate diphosphohydrolase 5 (ENTPD5), also known as CD39 antigen-like 4, ER-UDPase, Guanosine-diphosphatase ENTPD5, Nucleoside diphosphatase Uridine-diphosphatase ENTPD5. This hydrolase is expressed in response to phosphoinositide 3-kinase (PI3K) signaling. Activation of PI3K results in FOXO phosphorylation by AKT1 and loss of ENTPD5 transcriptional repression. It is Up-regulated in PTEN-deficient cells. Uridine diphosphatase (UDPase) that promotes protein N-glycosylation and ATP level regulation.ENTPD5 promotes protein N-glycosylation and folding in the endoplasmic reticulum, as well as elevated ATP consumption in the cytosol via an ATP hydrolysis cycle. Together with CMPK1 and AK1, ENTPD5 constitutes an ATP hydrolysis cycle that converts ATP to AMP and results in a compensatory increase in aerobic glycolysis. ENTPD5 also hydrolyzes GDP and IDP but not any other nucleoside di-, mono- or triphosphates, nor thiamine pyrophosphate. This enzyme Plays a key role in the AKT1-PTEN signaling pathway by promoting glycolysis in proliferating cells in response to phosphoinositide 3-kinase (PI3K) signaling.
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References
- Villar J, et al. (2009) PCPH/ENTPD5 expression confers to prostate cancer cells resistance against cisplatin-induced apoptosis through protein kinase Calpha-mediated Bcl-2 stabilization. Cancer Res. 69(1): 102-10.
- Fang M, et al. (2010) The ER UDPase ENTPD5 promotes protein N-glycosylation, the Warburg effect, and proliferation in the PTEN pathway. Cell. 143(5): 711-24.
- Paez JG, et al. (2001) Identity between the PCPH proto-oncogene and the CD39L4 (ENTPD5) ectonucleoside triphosphate diphosphohydrolase gene. Int J Oncol. 19(6): 1249-54.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"