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Product Name
Anti-Enterovirus 71 VP1 antibody
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Description
Mouse monoclonal to Enterovirus 71 VP1
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Tested applications
MN
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Species reactivity
EV Enterovirus 71 VP4
Has cross-reactivity with EV71 genogroup C and A in cell assay
No cross-reactivity with Coxsackievirus type A16 in cell assay -
Isotype
Mouse IgG1
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Preparation
This antibody was obtained from a mouse immunized with a peptide (GSQVSTQRSGSHENSNSATEK)-KLH conjugate.
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Clonality
Monoclonal
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Formulation
0.2 μm filtered solution in PBS
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
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Applications
Microneutralizaiton (MN) - The EV71 VP4 protein neutralization activity of antibody is Measured by microneutralization assay in vitro.The Neutralization titer (IC50) is typically 6.25 µg/ml with EV71 genogroup C4 (100CCID50).
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Validations
Enterovirus 71 VP4 Neutralizing Antibody
The neutralization activity of EV71 VP4 Neutralizing Antibody is Measured by Microneutralization test in vitro. The cytopathic effect of RD cells (10E5/well) induced by 100CCID50 EV71 genogroup C4 is neutralized by increasing concentrations of anti EV71 Monoclonal Antibody (Catalog EV71 M001). The IC50 is typically 4-8 µg/mL. NA: No antibody added.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"