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Product Name
Anti-EIF3K antibody
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Description
Mouse monoclonal to EIF3K
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Tested applications
IF, ICC/IF
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Species reactivity
Human EIF3K
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Alternative names
M9 antibody; ARG134 antibody; PLAC24 antibody; PTD001 antibody; EIF3S12 antibody; HSPC029 antibody; MSTP001 antibody; PLAC-24 antibody; PRO1474 antibody; EIF3-p28 antibody; Eif3s12 antibody; 1200009C21Rik antibody; 1200009C21Rik antibody; ARG134 antibody; eIF-3 p25 antibody; eIF-3 p25 antibody; eIF-3 p28 antibody; Eif3k antibody; EIF3K antibody; EIF3-p28 antibody; EIF3S12 antibody; Eif3s12 antibody; eukaryotic translation initiation factor 3 antibody; eukaryotic translation initiation factor 3 antibody; eukaryotic translation initiation factor 3 subunit 12 antibody; eukaryotic translation initiation factor 3 subunit 12 antibody; eukaryotic translation initiation factor 3 subunit K antibody; eukaryotic translation initiation factor 3 subunit K antibody; HSPC029 antibody; M9 antibody; MSTP001 antibody; muscle specific antibody; muscle-specific gene M9 protein antibody; PLAC24 antibody; PLAC-24 antibody; PRO1474 antibody; PTD001 antibody; subunit 12 antibody; subunit 12 antibody; subunit K antibody; subunit K antibody
- Immunogen
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Isotype
Mouse IgG1
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Preparation
This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human EIF3K (rh EIF3K; Q9UBQ5; Ala2-Gln218). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
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Clonality
Monoclonal
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Formulation
0.2 μm filtered solution in PBS
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
ICC/IF: 10-25 μg/mL
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Validations
EIF3K Antibody, Mouse MAb, Immunofluorescence
Immunofluorescence staining of Human EIF3K in Hela cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with mouse anti-Human EIF3K monoclonal antibody (15 µg/ml) at 4℃ overnight. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody(green). Positive staining was localized to cytoplasm and nucleus.
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Background
EIF3K is a member of the eIF3 subunit K family. It is a component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S preinitiation complex (43S PIC). It stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. EIF3K is universally expressed in human tissues. It is distributed both in nucleus and cytoplasm. EIF3K is the smallest subunit of eIF3 and it interacts with a number of other subunits of eIF3 and the 40S ribosomal subunit.
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References
- Rual JF. et al., 2005, Nature. 437 (7062): 1173-8.
- Shen X. et al., 2004, FEBS Lett. 573 (1-3): 139-46.
- Wei Z. et al., 2004, J Biol Chem. 279 (33): 34983-90.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"