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Anti-eIF3B Rabbit antibody

Cat.#: 167347

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Special Price 441.3 USD

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Product Information

  • Product Name
    Anti-eIF3B Rabbit antibody
  • Documents
  • Description
    eIF3B Rabbit polyclonal antibody
  • Tested applications
    WB, ICC/IF, FC, IP
  • Species reactivity
    Human, Mouse, Rat
  • Alternative names
    PRT1; EIF3S9; EIF3-ETA; EIF3-P110; EIF3-P116 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    Antigen: A synthetic peptide of human eIF3B
  • Clonality
    Polyclonal
  • Formulation
    Supplied in 50nM Tris-Glycine(pH 7.4), 0.15M Nacl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
  • Storage instructions
    Store at -20°C. Stable for 12 months from date of receipt.
  • Applications

    WB: 1/2000-1/10000

    ICC/IF: 1/20-1/50

    FC: 1/20

    IP: 1/20-1/50

  • Validations

    Immunocytochemistry of eIF3B(green) in Hela cells using eIF3B Rabbit pAb at dilution 1/50, and DAPI(blue)

    Immunocytochemistry of eIF3B(green) in Hela cells using eIF3B Rabbit pAb at dilution 1/50, and DAPI(blue)

    Western blot detection of eIF3B in Raji,COS7,3T3,Hela cell lysates using eIF3B Rabbit pAb(1:1000 diluted).Predicted band size:93KDa.Observed band size:117KDa.

    Western blot detection of eIF3B in Raji,COS7,3T3,Hela cell lysates using eIF3B Rabbit pAb(1:1000 diluted).Predicted band size:93KDa.Observed band size:117KDa.

  • Background
    Swiss-Prot Acc.P55884.RNA-binding component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis (PubMed:9388245, PubMed:17581632, PubMed:25849773, PubMed:27462815). The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation (PubMed:9388245, PubMed:17581632). The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression (PubMed:25849773).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"