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Product Name
Anti-EGR1 antibody
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Description
Rabbit polyclonal to EGR1
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Tested applications
IF, ICC/IF
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Species reactivity
Human EGR1
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Alternative names
egr antibody; TIS8 antibody; Zenk antibody; Egr-1 antibody; NGFIA antibody; Zfp-6 antibody; ETR103 antibody; Krox-1 antibody; Krox24 antibody; NGF1-A antibody; NGFI-A antibody; Zif268 antibody; Krox-24 antibody; A530045N19Rik antibody; TIS8 antibody; AT225 antibody; G0S30 antibody; NGFI-A antibody; ZNF225 antibody; KROX-24 antibody; ZIF-268 antibody
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Isotype
Rabbit IgG
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Preparation
Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human EGR1, and purified by antigen affinity chromatography.
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Clonality
Polyclonal
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Formulation
0.2 μm filtered solution in PBS
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
ICC/IF: 0.5-1.5 μg/mL
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Validations
EGR1 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunofluorescence
Immunofluorescence staining of EGR1 in HeLa cells. Cells were fixed with 4% PFA,blocked with 10% serum. Then incubated with rabbit anti-human EGR1 polyclonal antibody (1 µg/ml)(Figure A)，incubated with rabbit anti-human EGR1 polyclonal antibody and antigen (Figure B) at 4℃ overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm and nucleus.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"