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Product Name
Anti-EGFR/HER1/ErbB1 antibody
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Description
Rabbit polyclonal to EGFR/HER1/ErbB1
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Tested applications
ELISA, WB, IP
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Species reactivity
Rat EGFR / HER1 / ErbB1
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Alternative names
ERBB antibody; HER1 antibody; mENA antibody; ERBB1 antibody; PIG61 antibody; NISBD2 antibody; Wa5 antibody; wa2 antibody; Erbb antibody; Errp antibody; wa-2 antibody; Errb1 antibody; AI552599 antibody; 9030024J15Rik antibody; 9030024J15Rik antibody; AI552599 antibody; EGFR antibody; Egfr antibody; Egfr antibody; ERBB antibody; Erbb antibody; ERBB1 antibody; ERBB1 antibody; ErbB-1 antibody; Errp antibody; Errp antibody; HER1 antibody; mENA antibody; PIG61 antibody; RP23-295E4.1 antibody; wa2 antibody; wa-2 antibody; Wa5 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
Produced in rabbits immunized with purified, recombinant Rat EGFR / HER1 / ErbB1 (rh EGFR / HER1 / ErbB1; E7CXR8; Met1-Ser646). EGFR / HER1 / ErbB1 specific IgG was purified by Rat EGFR / HER1 / ErbB1 affinity chromatography.
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Clonality
Polyclonal
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Formulation
0.2 μm filtered solution in PBS
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
WB: 10-20 μg/ml
ELISA: 0.1-0.2 μg/mL
This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Rat EGFR / HER1 / ErbB1. The detection limit for Rat EGFR / HER1 / ErbB1 is 0.00245 ng/well.
IP: 1-4 μg/mg of lysate
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Validations
EGFR / HER1 / ErbB1 Antibody, Rabbit PAb, Antigen Affinity Purified, Western blot
EGFR / HER1 / ErbB1 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunoprecipitation
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Background
As a member of the epidermal growth factor receptor (EGFR) family, EGFR protein is type I transmembrane glycoprotein that binds a subset of EGF family ligands including EGF, amphiregulin, TGF-α, betacellulin, etc. EGFR protein plays a crucial role in signaling pathway in the regulation of cell proliferation, survival and differentiation. Binding of a ligand induces EGFR protein homo- or heterodimerization, the subsequent tyrosine autophosphorylation and initiates various down stream pathways (MAPK, PI3K/PKB and STAT). In addition, EGFR signaling also has been shown to exert action on carcinogenesis and disease progression, and thus EGFR protein is proposed as a target for cancer therapy currently.
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References
- Schlessinger, J. (2000) Cell signaling by receptor tyrosine kinases. Cell 103(2): 211-25.
- Giaccone, G. (2005) HER1/EGFR-targeted agents: predicting the future for patients with unpredictable outcomes to therapy. Ann. Oncol. 16(4): 538-48.
- Yarden, Y., et al. (2001) Untangling the ErbB signalling network. Nat. Rev. Mol. Cell. Biol. 2(2): 127-37.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"