Anti-Eg5 (4H3) Mouse antibody

Cat.#: 168091

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Product Information

  • Product Name
    Anti-Eg5 (4H3) Mouse antibody
  • Documents
  • Description
    Eg5 (4H3) Mouse monoclonal antibody
  • Tested applications
    WB, IP, ICC/IF
  • Species reactivity
    Human
  • Alternative names
    EG5;HKSP;KIF11;KIF11_HUMAN;kinesin family member 11;Kinesin like protein 1;Kinesin like spindle protein HKSP;Kinesin-like protein 1;Kinesin-like protein KIF11;Kinesin-like spindle protein HKSP;Kinesin-related motor protein Eg5;KNSL1;MCLMR;Thyroid receptor antibody
  • Isotype
    Mouse IgG1
  • Preparation
    Antigen: Purified recombinant human Eg5 protein fragments expressed in E.coli.
  • Clonality
    Monoclonal
  • Formulation
    Purified mouse monoclonal antibody in PBS(pH 7.4) containing with 0.02% sodium azide and 50% glycerol.
  • Storage instructions
    Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Applications

    WB: 1/1000

    ;ICC: 1/200

  • Validations

    Western blot detection of Eg5 in MCF7,293T,Jurkat and Hela cell lysates using Eg5 mouse mAb (1:1000 diluted).Predicted band size:130KDa.Observed band size:130KDa.

    Western blot detection of Eg5 in MCF7,293T,Jurkat and Hela cell lysates using Eg5 mouse mAb (1:1000 diluted).Predicted band size:130KDa.Observed band size:130KDa.

    Immunoprecipitation analysis of Jurkat cell lysates using Eg5 mouse mAb.

    Immunoprecipitation analysis of Jurkat cell lysates using Eg5 mouse mAb.

    Immunocytochemistry staining of Hela using Eg5 mouse mAb (1:200).

    Immunocytochemistry staining of Hela using Eg5 mouse mAb (1:200).

  • Background
    Swiss-Prot Acc.P52732.Motor protein required for establishing a bipolar spindle. Blocking of KIF11 prevents centrosome migration and arrest cells in mitosis with monoastral microtubule arrays.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"