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Product Name
Anti-Eg5 (4H3) Mouse antibody
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Description
Eg5 (4H3) Mouse monoclonal antibody
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Tested applications
WB, IP, ICC/IF
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Species reactivity
Human
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Alternative names
EG5;HKSP;KIF11;KIF11_HUMAN;kinesin family member 11;Kinesin like protein 1;Kinesin like spindle protein HKSP;Kinesin-like protein 1;Kinesin-like protein KIF11;Kinesin-like spindle protein HKSP;Kinesin-related motor protein Eg5;KNSL1;MCLMR;Thyroid receptor antibody
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Isotype
Mouse IgG1
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Preparation
Antigen: Purified recombinant human Eg5 protein fragments expressed in E.coli.
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Clonality
Monoclonal
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Formulation
Purified mouse monoclonal antibody in PBS(pH 7.4) containing with 0.02% sodium azide and 50% glycerol.
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/1000
;ICC: 1/200
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Validations
Western blot detection of Eg5 in MCF7,293T,Jurkat and Hela cell lysates using Eg5 mouse mAb (1:1000 diluted).Predicted band size:130KDa.Observed band size:130KDa.
Immunoprecipitation analysis of Jurkat cell lysates using Eg5 mouse mAb.
Immunocytochemistry staining of Hela using Eg5 mouse mAb (1:200).
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Background
Swiss-Prot Acc.P52732.Motor protein required for establishing a bipolar spindle. Blocking of KIF11 prevents centrosome migration and arrest cells in mitosis with monoastral microtubule arrays.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"