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Anti-E2F2 antibody

Cat.#: 176530

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Special Price 241.9 USD

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Product Information

  • Product Name
    Anti-E2F2 antibody
  • Documents
  • Description
    Rabbit monoclonal antibody to E2F2
  • Tested applications
    WB, FC
  • Species reactivity
    Human, Mouse, Rat
  • Alternative names
    E2F-2 antibody
  • Isotype
    Rabbit IgG
  • Preparation
    This antigen of this antibody was recombinant protein within human e2f2 aa 300-437.
  • Clonality
    Monoclonal
  • Formulation
    Liquid, 1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
  • Storage instructions
    Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
  • Applications

    WB: 1:1,000-1:2,000

    FC: 1:50-1:100

  • Validations

    Fig1:; Western blot analysis of E2F2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody ( 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.; Positive control:; Lane 1: K562 cell lysate; Lane 2: 293T cell lysate; Lane 3: HepG2 cell lysate

    Fig1:; Western blot analysis of E2F2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody ( 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:200,000 dilution was used for 1 hour at room temperature.; Positive control:; Lane 1: K562 cell lysate; Lane 2: 293T cell lysate; Lane 3: HepG2 cell lysate

    Fig2:; Flow cytometric analysis of E2F2 was done on K562 cells. The cells were fixed, permeabilized and stained with the primary antibody ( 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    Fig2:; Flow cytometric analysis of E2F2 was done on K562 cells. The cells were fixed, permeabilized and stained with the primary antibody ( 1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

  • Background
    Q14209
  • References
    • Mei Z et al. FBXO32 Targets c-Myc for Proteasomal Degradation and Inhibits c-Myc Activity. J Biol Chem 290:16202-14 (2015).
    • Perry MC et al. ERBB2 deficiency alters an E2F-1-dependent adaptive stress response and leads to cardiac dysfunction. Mol Cell Biol 34:4232-43 (2014).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"