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Product Name
Anti-E-tag antibody
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Description
Rabbit Polyclonal to E-tag
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Tested applications
WB, ICC/IF, IP
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Species reactivity
Recognize N-terminal and C-terminal E tag in fusion proteins
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Isotype
Rabbit IgG
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Preparation
Produced in rabbits immunized with A synthetic peptide corresponding to the E-tag sequence (GAPVPYPDPLEPR), and purified by antigen affinity chromatography.
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Clonality
Polyclonal
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Formulation
0.2 μm filtered solution in PBS
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
WB: 0.1-5 μg/ml
ICC/IF: 0.1-1.0 μg/mL
IP: 0.5-2 μg/mg of lysate
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Validations
Anti-E-tag Antibody, Rabbit Pab, Antigen Affinity Purified， Western Blot
Anti-E-tag Antibody, Rabbit Pab, Antigen Affinity Purified, Immunohistochemistry
Immunofluorescence staining of E-Tag in Hela cells, transfected with pSTEP2-Etag-GFERh (Figure A), pSTEP2-his-Etag-GFER (Figure B), pSTEP2-his-GFER-Etag (Figure C), pSTEP2-Etag-GSTT2h (Figure D),pSTEP2-his-Etag-GSTT2 (Figure E) and pSTEP2-his-GSTT2-Etag (Figure F). Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-E-Tag monoclonal antibody at 37℃ 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI (blue).
Anti-E-tag Antibody, Rabbit Pab, Antigen Affinity Purified, Immunoprecipitation
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"