Anti-E-tag antibody

Cat.#: 100291

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Product Information

  • Product Name
    Anti-E-tag antibody
  • Documents
  • Description
    Rabbit Polyclonal to E-tag
  • Tested applications
    WB, ICC/IF, IP
  • Species reactivity
    Recognize N-terminal and C-terminal E tag in fusion proteins
  • Isotype
    Rabbit IgG
  • Preparation
    Produced in rabbits immunized with A synthetic peptide corresponding to the E-tag sequence (GAPVPYPDPLEPR), and purified by antigen affinity chromatography.
  • Clonality
    Polyclonal
  • Formulation
    0.2 μm filtered solution in PBS
  • Storage instructions
    This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
    Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
  • Applications

    WB: 0.1-5 μg/ml

    ICC/IF: 0.1-1.0 μg/mL

    IP: 0.5-2 μg/mg of lysate

  • Validations

    Anti-E-tag Antibody, Rabbit Pab, Antigen Affinity Purified, Western Blot

    Anti-E-tag Antibody, Rabbit Pab, Antigen Affinity Purified, Western Blot

    Anti-E-tag Antibody, Rabbit Pab, Antigen Affinity Purified, Immunohistochemistry

    Anti-E-tag Antibody, Rabbit Pab, Antigen Affinity Purified, Immunohistochemistry

    Immunofluorescence staining of E-Tag in Hela cells, transfected with pSTEP2-Etag-GFERh (Figure A), pSTEP2-his-Etag-GFER (Figure B), pSTEP2-his-GFER-Etag (Figure C), pSTEP2-Etag-GSTT2h (Figure D),pSTEP2-his-Etag-GSTT2 (Figure E) and pSTEP2-his-GSTT2-Etag (Figure F). Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS, blocked with 10% serum, and incubated with Mouse anti-E-Tag monoclonal antibody at 37℃ 1 hour. Then cells were stained with the Alexa Fluor® 488-conjugated Goat Anti-mouse IgG secondary antibody (green) and counterstained with DAPI (blue).

    Anti-E-tag Antibody, Rabbit Pab, Antigen Affinity Purified, Immunoprecipitation

    Anti-E-tag Antibody, Rabbit Pab, Antigen Affinity Purified, Immunoprecipitation

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"