Anti-DYKDDDDK-tag Mouse mAb(HRP-Conjugate)

Cat.#: 169089

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Product Information

  • Product Name
    Anti-DYKDDDDK-tag Mouse mAb(HRP-Conjugate)
  • Documents
  • Description
    DYKDDDDK-tag Mouse mAb(HRP-Conjugate)
  • Tested applications
    WB, IP
  • Species reactivity
    Tag
  • Alternative names
    FLAG antibody
  • Isotype
    Mouse IgG2b
  • Preparation
    Antigen: Monoclonal antibodies are produced by immunizing mouse with synthetic peptide (DYKDDDDK), coupled to KLH.
  • Clonality
    Monoclonal
  • Formulation
    In PBS(pH 7.4) containing with 1mg/ml BSA 0.03% Proclin300 and 50% glycerol.
  • Storage instructions
    Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Applications

    WB: 1/1000

  • Validations

    Western blot analysis of extracts from CHO-K1 cells, untransfected (A) or transfected with different FLAG-fusion proteins (B,C,D), using DYKDDDDK-tag-HRP (HRP-Conjugate) Mouse mAb (1:1000 diluted).

    Western blot analysis of extracts from CHO-K1 cells, untransfected (A) or transfected with different FLAG-fusion proteins (B,C,D), using DYKDDDDK-tag-HRP (HRP-Conjugate) Mouse mAb (1:1000 diluted).

  • Background
    Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein’s biochemical properties.The DYKDDDDK peptide has been used extensively as a general epitope tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"