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Product Name
Anti-DPY30 antibody
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Description
Rabbit polyclonal antibody to DPY30
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Tested applications
ICC, IHC-P
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Species reactivity
Human, Mouse
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Alternative names
Cps25 antibody; Saf19 antibody; HDPY-30 antibody
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Isotype
IgG
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Preparation
This antigen of this antibody was recombinant protein
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Clonality
Polyclonal
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Formulation
Liquid, 1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
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Storage instructions
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
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Applications
IHC-P: 1:100-1:200
ICC: 1:100-1:200
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Validations
Fig1: ICC staining DPY30 in F9 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS and counterstained with DAPI in order to highlight the nucleus (blue).
Fig2: ICC staining DPY30 in D3 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS and counterstained with DAPI in order to highlight the nucleus (blue).
Fig3: Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using anti- DPY30 rabbit polyclonal antibody.
Fig4: Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-DPY30 rabbit polyclonal antibody.
- Background
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References
- Crystal structure of the C-terminal domain of human DPY-30-like protein: A component of the histone methyltransferase complex. Wang X., Lou Z., Dong X., Yang W., Peng Y., Yin B., Gong Y., Yuan J., Zhou W., Bartlam M., Peng X., Rao Z. J. Mol. Biol. 390:530-537(2009)
- A role of histone H3 lysine 4 methyltransferase components in endosomal trafficking. Xu Z., Gong Q., Xia B., Groves B., Zimmermann M., Mugler C., Mu D., Matsumoto B., Seaman M., Ma D. J. Cell Biol. 186:343-353(2009)
- Role for Dpy-30 in ES cell-fate specification by regulation of H3K4 methylation within bivalent domains. Jiang H., Shukla A., Wang X., Chen W.Y., Bernstein B.E., Roeder R.G. Cell 144:513-525(2011)
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"