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Product Name
Anti-DKK3 antibody
- Documents
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Description
Rabbit polyclonal to DKK3
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Tested applications
ELISA, IHC-P
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Species reactivity
Mouse Dkk-3 / Dickkopf-3 / DKK3
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Alternative names
hDKK 3 antibody; hDKK3 antibody; REIC antibody; RIG antibody; RIG antibody; REIC antibody; C87148 antibody; AW061014 antibody; Dickkopf 3 antibody; Dickkopf3 antibody; DKK3 antibody; DKK-3 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
Produced in rabbits immunized with purified, recombinant Mouse Dkk3 / Dickkopf-3 (rM Dkk3; NP_056629.1; Met 1-Ile 349). Dkk3 specific IgG was purified by mouse Dkk3 affinity chromatography
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Clonality
Polyclonal
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Formulation
0.2 μm filtered solution in PBS with 5% trehalose
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
ELISA: 0.5-1.0 μg/mL
This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect Mouse Dkk-3. The detection limit for Mouse Dkk-3 is 0.00245 ng/well.
IHC-P: 0.1-1 μg/mL
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Validations
DKK-3 / Dkk3 / Dickkopf-3 Antibody, Rabbit PAb, Antigen Affinity Purified
Immunochemical staining of mouse DKK3 in mouse brain with rabbit polyclonal antibody (0.2 µg/mL, formalin-fixed paraffin embedded sections).
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Background
DKK3 (dickkopf related protein 3) is a member of the dickkopf-related family consisting of DKK1, DKK2, DKK3 and DKK4. It is a secreted protein, and also known as REIC (Reduced Expansion in Immortalized Cells). The DKK3 protein is proposed to function as a secreted tumor suppressor since it is downregulated in a number of cancer cells and prostate cancer tissue and may be a promising candidate molecule for therapeutic interference. DKK3 protein is also a negative regulator of beta-catenin and its downregulation contribute to an activation of the beta-catenin signaling pathway.
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References
- Yue W, et al., 2008. Downregulation of Dkk3 activates beta-catenin/TCF-4 signaling in lung cancer. Carcinogenesis, 29 (1): 84-92.
- Ding Z, et al., 2009. Promoter methylation and mRNA expression of DKK3 and WIF-1 in hepatocellular carcinoma. World J Gastroenterol, 15 (21):2595-601.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"