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Product Name
Anti-DCUN1D1 / SCCRO antibody
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Description
Rabbit polyclonal to DCUN1D1 / SCCRO
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Tested applications
ELISA, WB, IP
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Species reactivity
Human DCUN1D1 / SCCRO
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Alternative names
DCN1 antibody; defective in cullin neddylation 1 antibody; domain containing 1 (S. cerevisiae) antibody; DCUN1D1 antibody; DCNL1 antibody; DCUN1L1 antibody; RP42 antibody; SCCRO antibody; SCRO antibody; Tes3 antibody; DCN1-like protein 1 antibody; DCUN1 domain-containing protein 1 antibody; RP42 homolog antibody; defective in cullin neddylation protein 1-like protein 1 antibody; squamous cell carcinoma-related oncogene antibody; DCN1 antibody; defective in cullin neddylation 1 antibody; domain containing 1 (S. cerevisiae) antibody; Dcun1d1 antibody; Rp42 antibody; SCCRO antibody; Tes3 antibody; pTes3 antibody; DCN1-like protein 1 antibody; DCUN1 domain-containing protein 1 antibody; defective in cullin neddylation protein 1-like protein 1 antibody; testis derived transcript 3 antibody; testis-specific protein 3 antibody; RP42 antibody; SCRO antibody; Tes3 antibody; DCNL1 antibody; SCCRO antibody; DCUN1L1 antibody; Rp42 antibody; Tes3 antibody; SCCRO antibody; pTes3 antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
Produced in rabbits immunized with purified, recombinant Human DCUN1D1 / SCCRO (rh DCUN1D1 / SCCRO; NP_065691.2; Met1-Val259). DCUN1D1 / SCCRO specific IgG was purified by Human DCUN1D1 / SCCRO affinity chromatography.
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Clonality
Polyclonal
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Formulation
0.2 μm filtered solution in PBS
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
WB: 5-10 μg/ml
ELISA:0.1-0.2 μg/mL
This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human DCUN1D1 / SCCRO. The detection limit for Human DCUN1D1 / SCCRO is < 0.039 ng/well.
IP: 1-4 μg/mg of lysate
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Validations
DCUN1D1 / SCCRO Antibody, Rabbit PAb, Antigen Affinity Purified, Western blot
DCUN1D1 / SCCRO Antibody, Rabbit PAb, Antigen Affinity Purified, Immunoprecipitation
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Background
DCUN1D1, also known as SCCRO, is part of an E3 ubiquitin ligase complex for neddylation. DCUN1D1 functions to recruit charged E2 and is involved in the release of inhibitory effects of CAND1 on cullin-RING ligase E3 complex assembly and activity. DCUN1D1 binds to the components of the neddylation pathway (Cullin-ROC1, Ubc12, and CAND1) and augments but is not required for cullin neddylation in reactions using purified recombinant proteins. DCUN1D1 also recruits Ubc12 approximately NEDD8 to the CAND1-Cul1-ROC1 complex but that this is not sufficient to dissociate or overcome the inhibitory effects of CAND1 on cullin neddylation in purified protein assays. DCUN1D1 also acts as am ncogene facilitating malignant transformation and carcinogenic progression.
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References
- Heir P. et al., 2013, Mol Cell Biol. 33 (8): 1621-31.
- Kurz T. et al., 2005, Nature. 435 (7046): 1257-61.
- Kim AY. et al., 2008, J Biol Chem. 283 (48): 33211-20.
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