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Anti-DCTN4 antibody

Cat.#: 175056

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Product Information

  • Product Name
    Anti-DCTN4 antibody
  • Documents
  • Description
    Mouse monoclonal antibody to DCTN4
  • Tested applications
    WB, ICC, IHC-P, FC
  • Species reactivity
    Human
  • Alternative names
    P62 antibody; DYN4 antibody
  • Isotype
    IgG1
  • Preparation
    This antigen of this antibody was recombinant protein
  • Clonality
    Monoclonal
  • Formulation
    Liquid, 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
  • Storage instructions
    Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
  • Applications

    WB: 1:500-1:1,000

    ICC: 1:100-1:500

    IHC-P: 1:100-1:500

    FC: 1:100-1:200

  • Validations

    Fig1: Western blot analysis of DCTN4 on human DCTN4 recombinant protein using anti-DCTN4 antibody at 1/1,000 dilution.

    Fig1: Western blot analysis of DCTN4 on human DCTN4 recombinant protein using anti-DCTN4 antibody at 1/1,000 dilution.

    Fig2: Western blot analysis of DCTN4 on HEK293 (1) and DCTN4-hIgGFc transfected HEK293 (2) cell lysate using anti-DCTN4 antibody at 1/1,000 dilution.

    Fig2: Western blot analysis of DCTN4 on HEK293 (1) and DCTN4-hIgGFc transfected HEK293 (2) cell lysate using anti-DCTN4 antibody at 1/1,000 dilution.

    Fig3: Western blot analysis of DCTN4 on Raw264.7 (1) and NIH3T3 (2) cell lysate using anti-DCTN4 antibody at 1/1,000 dilution.

    Fig3: Western blot analysis of DCTN4 on Raw264.7 (1) and NIH3T3 (2) cell lysate using anti-DCTN4 antibody at 1/1,000 dilution.

    Fig4: ICC staining DCTN4 (green) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    Fig4: ICC staining DCTN4 (green) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

    Fig5: Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue using anti-DCTN4 antibody. Counter stained with hematoxylin.

    Fig5: Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue using anti-DCTN4 antibody. Counter stained with hematoxylin.

    Fig6: Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue using anti-DCTN4 antibody. Counter stained with hematoxylin.

    Fig6: Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue using anti-DCTN4 antibody. Counter stained with hematoxylin.

    Fig7: Flow cytometric analysis of A549 cells with DCTN4 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).

    Fig7: Flow cytometric analysis of A549 cells with DCTN4 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).

  • Background
    Q9UJW0
  • References
    • Ayalon G et al. An ankyrin-based mechanism for functional organization of dystrophin and dystroglycan. Cell 135(7):1189-200 (2008).
    • Lim CM et al. Copper-dependent interaction of dynactin subunit p62 with the N terminus of ATP7B but not ATP7A. J Biol Chem 281(20):14006-14 (2006).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"