-
Product Name
Anti-DCTN4 antibody
- Documents
-
Description
Mouse monoclonal antibody to DCTN4
-
Tested applications
WB, ICC, IHC-P, FC
-
Species reactivity
Human
-
Alternative names
P62 antibody; DYN4 antibody
-
Isotype
Mouse IgG1
-
Preparation
This antigen of this antibody was recombinant protein
-
Clonality
Monoclonal
-
Formulation
Liquid, 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
-
Storage instructions
Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
-
Applications
WB: 1:500-1:1,000
ICC: 1:100-1:500
IHC-P: 1:100-1:500
FC: 1:100-1:200
-
Validations
Fig1: Western blot analysis of DCTN4 on human DCTN4 recombinant protein using anti-DCTN4 antibody at 1/1,000 dilution.
Fig2: Western blot analysis of DCTN4 on HEK293 (1) and DCTN4-hIgGFc transfected HEK293 (2) cell lysate using anti-DCTN4 antibody at 1/1,000 dilution.
Fig3: Western blot analysis of DCTN4 on Raw264.7 (1) and NIH3T3 (2) cell lysate using anti-DCTN4 antibody at 1/1,000 dilution.
Fig4: ICC staining DCTN4 (green) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig5: Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue using anti-DCTN4 antibody. Counter stained with hematoxylin.
Fig6: Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue using anti-DCTN4 antibody. Counter stained with hematoxylin.
Fig7: Flow cytometric analysis of A549 cells with DCTN4 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).
- Background
-
References
- Ayalon G et al. An ankyrin-based mechanism for functional organization of dystrophin and dystroglycan. Cell 135(7):1189-200 (2008).
- Lim CM et al. Copper-dependent interaction of dynactin subunit p62 with the N terminus of ATP7B but not ATP7A. J Biol Chem 281(20):14006-14 (2006).
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"