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Product Name
Anti-DBF4 Rabbit antibody
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Description
DBF4 Rabbit monoclonal antibody
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Tested applications
WB
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Species reactivity
Human
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Alternative names
ASK; CHIF; DBF4A; ZDBF1 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthetic peptide of human DBF4
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Clonality
Monoclonal
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Formulation
50nM Tris-Glycine(pH 7.4), 0.15M Nacl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
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Storage instructions
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
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Applications
WB: 1/2000-1/10000
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Validations
Western blot detection of DBF4 in Hela,Jurkat cell lysates using DBF4 Rabbit mAb(1:500 diluted).Predicted band size:77kDa.Observed band size:77kDa.
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Background
Swiss-Prot Acc.Q9UBU7.Regulatory subunit for CDC7 which activates its kinase activity thereby playing a central role in DNA replication and cell proliferation. Required for progression of S phase. The complex CDC7-DBF4A selectively phosphorylates MCM2 subunit at 'Ser-40' and 'Ser-53' and then is involved in regulating the initiation of DNA replication during cell cycle.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"