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Product Name
Anti-CRIP2 antibody
- Documents
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Description
Rabbit polyclonal to CRIP2
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Tested applications
ELISA, WB, IHC-P
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Species reactivity
Human CRIP2
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Alternative names
ESP1 antibody; Esp1 antibody; 0610010I23Rik antibody; AW743261 antibody; C77570 antibody; CRIP antibody; Crip2 antibody; CRIP2 antibody; CRP2 antibody; CRP2 antibody; CRP-2 antibody; CRP4 antibody; cysteine rich protein 2 antibody; Cysteine-rich intestinal protein antibody; cysteine-rich protein 2 antibody; ESP1 antibody; ESP1 antibody; heart LIM protein antibody; Hlp antibody; LIM domain protein ESP1/CRP2 antibody; LIM only protein HLP antibody; CRP antibody; CRIP antibody; CRP2 antibody; Crp antibody; Hlp antibody; CRP2 antibody; CRP4 antibody; C77570 antibody; AW743261 antibody; 0610010I23Rik antibody
- Immunogen
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Isotype
Rabbit IgG
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Preparation
Produced in rabbits immunized with purified, recombinant Human CRIP2 (rh CRIP2; P52943; Met1-Pro208). CRIP2 specific IgG was purified by Human CRIP2 affinity chromatography.
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Clonality
Polyclonal
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Formulation
0.2 μm filtered solution in PBS
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Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
WB: 2-5 μg/ml
ELISA:0.1-0.2 μg/mL
This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human CRIP2. The detection limit for Human CRIP2 is 0.00245 ng/well.
IHC-P: 0.1-2 μg/ml
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Validations
CRIP2 Antibody, Rabbit PAb, Antigen Affinity Purified, Western blot
CRIP2 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
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Background
CRIP2 is a putative transcription factor. It has a widespread tissue expression and is highly expressed in heart. CRIP2 contains two LIM zinc-binding domains. CRIP2 may participate in the differentiation of smooth muscle tissue. It also plays an important role in esophageal squamous cell carcinoma (ESCC) tumorigenesis. CRIP2 acts as a transcription repressor of the NF-κB-mediated proangiogenic cytokine expression and thus functionally inhibits tumor formation and angiogenesis. It interacts with the NF-κB/p65 to inhibit its DNA-binding ability to the promoter regions of the major proangiogenesis cytokines critical for tumor progression, including IL6, IL8, and VEGF. In conclusion, we provide compelling evidence that CRIP2 acts as a transcription repressor of the NF-κB-mediated proangiogenic cytokine expression and thus functionally inhibits tumor formation and angiogenesis.
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References
- Chang DF. et al., 2003, Dev Cell. 4 (1):107-18.
- Huber A. et al., 2000, J Biol Chem. 275 (8): 5504-11.
- Karim MA. et al., 1996, Genomics. 31 (2): 167-76.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"