-
Product Name
Anti-CRELD1 antibody
- Documents
-
Description
Rabbit polyclonal to CRELD1
-
Tested applications
ELISA, IHC-P
-
Species reactivity
Human CRELD1
-
Alternative names
AI843811 antibody; AVSD2 antibody; CIRRIN antibody; cysteine-rich with EGF-like domain protein 1 antibody; cysteine-rich with EGF-like domain protein 1 antibody; i11E7 protein antibody; UNQ188/PRO214 antibody; AVSD2 antibody; CIRRIN antibody; AI843811 antibody
- Immunogen
-
Isotype
Rabbit IgG
-
Preparation
Produced in rabbits immunized with purified, recombinant Human CRELD1 (rh CRELD1; NP_001070883.1; Met1-Glu362). CRELD1 specific IgG was purified by Human CRELD1 affinity chromatography.
-
Clonality
Polyclonal
-
Formulation
0.2 μm filtered solution in PBS with 5% trehalose
-
Storage instructions
This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.
Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. -
Applications
WB: 10-20 μg/ml
ELISA: 0.1-0.2 μg/mL
This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Human CRELD1. The detection limit for Human CRELD1 is < 0.039 ng/well.
IHC-P: 0.1-2 μg/ml
-
Validations
CRELD1 Antibody, Rabbit PAb, Antigen Affinity Purified, Western blot
CRELD1 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
Immunochemical staining of human CRELD1 in human brain with rabbit polyclonal antibody (1 µg/mL, formalin-fixed paraffin embedded sections).
CRELD1 Antibody, Rabbit PAb, Antigen Affinity Purified, Immunohistochemistry
Immunochemical staining of human CRELD1 in human kidney with rabbit polyclonal antibody (1 µg/mL, formalin-fixed paraffin embedded sections).
-
Background
CRELD1 is a transmembrane glycoprotein. Epidermal growth factor(EGF)like domain exists in CRELD1. EGF-like repeats are a class of cysteine-rich domains that mediate interactions between proteins of diverse function. EGF domains are found in proteins that are either completely secreted or have transmembrane regions that tether the protein to the cell surface. CRELD1 contains a 333 amino acid acid (aa) extracellular domain (ECD), two tandem transmembrane segments, and a second ECD of 15 aa. Defects in CRELD1 may cause susceptibility to atrioventricular septal defect type 2 which results in a persistent common atrioventricular canal.
-
References
- Robinson SW, et al. (2003) Missense Mutations in CRELD1 Are Associated with Cardiac Atrioventricular Septal Defects. Am J Hum Genet. 72(4):1047-52.
- Zatyka M, et al. (2005) Analysis of CRELD1 as a candidate 3p25 atrioventicular septal defect locus (AVSD2). Clin Genet. 67(6):526-8.
- Stelzl U, et al. (2005) A human protein-protein interaction network: a resource for annotating the proteome. Cell. 122(6):957-68.
Related Products / Services
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"