Anti-COX IV (4D11) Mouse antibody
COX IV (4D11) Mouse monoclonal antibody
WB, FC, ICC/IF, IP, IHC-P
Human, Mouse, Rat, Hamster, Goat, Monkey
AL024441;COX 4;COX IV 1;COX IV;COX IV-1;Cox4;COX41_HUMAN;Cox4a;COX4B;COX4I1;COX4I2; COX4L2;COXIV;Cytochrome c oxidase polypeptide IV;Cytochrome c oxidase subunit 4 isoform 1 mitochondrial;Cytochrome c oxidase subunit 4 isoform 1 antibody; mitochondrial;Cytochrome antibody
Antigen: A synthetic peptide corresponding to carboxyl terminal residues of human COX IV
Purified mouse monoclonal antibody in buffer containing 0.1M Tris-Glycine(pH 7.4 150 mM NaCl)with 0.02% sodium azide 0.1mg/mlBSA and 50% glycerol.
Store at 4°C short term. Store at -20°C long term. Avoid freeze / thaw cycle.
Western blot detection of COX IV in Mouse skeletal muscel and Jurkat lysates using COX IV mouse mAb (1:1000 diluted). Predicted band size: 17KDa.Observed band size: 17KDa.
Flow Cytometry analysis of K562 cells stained with COX4 (red, 1/100 dilution), followed by FITC-conjugated goat anti-mouse IgG. Blue line histogram represents the isotype control, normal mouse IgG.
Immunohistochemical analysis of paraffin-embedded human colorectal carcinoma with COX IV Mouse mAb (4D11-B3-E8,1:50 diluted),showing cytoplasm localization.A high pressure mediated antigen retrieval step was performed in citrate buffer(pH6.0).
Immunoprecipitation analysis of Hela cell lysates using COX IV mouse mAb.
Immunocytochemistry of HeLa cells using anti-COX IV mouse mAb diluted 1:150.
Western blot detection of COX IV in Goat muscle,CHO-k1,COS7,3T3,Hela,C6 and K562 cell lysates using COX IV mouse mAb (1:5000 diluted).Predicted band size:17KDa.Observed band size:17KDa.
Swiss-Prot Acc.P13073.This protein is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport.
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