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Product Name
Anti-Cortactin Rabbit antibody
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Description
Cortactin Rabbit polyclonal antibody
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Tested applications
WB, IHC-P, ICC/IF, FC, IP
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Species reactivity
Human, Mouse, Rat
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Alternative names
EMS1 antibody
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Isotype
Rabbit IgG
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Preparation
Antigen: A synthetic peptide of human Cortactin
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Clonality
Polyclonal
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Formulation
Supplied in 50nM Tris-Glycine(pH 7.4), 0.15M Nacl, 40%Glycerol, 0.01% sodium azide and 0.05% BSA.
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Storage instructions
Store at -20°C. Stable for 12 months from date of receipt.
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Applications
WB: 1/5000-1/10000
IHC: 1/20-1/100
ICC/IF: 1/200
FC: 1/20
IP: 1/20
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Validations
Immunocytochemistry of Cortactin(green) in Hela cells using Cortactin Rabbit pAb at dilution 1/50, and DAPI(blue)
Immunohistochemistry of Cortactin in paraffin-embedded Human breast cancer tissue using Cortactin Rabbit pAb at dilution 1/20
Western blot detection of Cortactin in K562,Rat Brain,C6,3T3,Hela cell lysates using Cortactin Rabbit pAb(1:1000 diluted).Predicted band size:62kDa.Observed band size:80-85kDa.
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Background
Swiss-Prot Acc.Q14247.Contributes to the organization of the actin cytoskeleton and cell shape (PubMed:21296879). Plays a role in the formation of lamellipodia and in cell migration. Plays a role in the regulation of neuron morphology, axon growth and formation of neuronal growth cones . Through its interaction with CTTNBP2, involved in the regulation of neuronal spine density . Plays a role in the invasiveness of cancer cells, and the formation of metastases (PubMed:16636290). Plays a role in focal adhesion assembly and turnover . In complex with ABL1 and MYLK regulates cortical actin-based cytoskeletal rearrangement critical to sphingosine 1-phosphate (S1P)-mediated endothelial cell (EC) barrier enhancement (PubMed:20861316). Plays a role in intracellular protein transport and endocytosis, and in modulating the levels of potassium channels present at the cell membrane (PubMed:17959782). Plays a role in receptor-mediated endocytosis via clathrin-coated pits . Required for stabilization of KCNH1 channels at the cell membrane (PubMed:23144454).
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